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ALK Activation Antibody Kit | ALK antibody kit
ALK Activation Antibody Sampler Kit
Applications
Western Blot
Synonyms
ALK Activation; ALK Activation Antibody Sampler Kit; ALK antibody kit
Applicable Applications for ALK antibody kit
Western Blot (WB)
Western Blot (WB)
(Western Blot analysis of 453 cells with Phospho-JAK3 (Tyr785) Polyclonal Antibody at dilution of 1:1000)
Western Blot (WB)
(Western Blot analysis of 3T3 cells with Phospho-JAK2 (Tyr1007) Polyclonal Antibody at dilution of 1:1000)
Western Blot (WB)
(Western Blot analysis of KB, MCF7 cells with Phospho-Stat3 (Tyr705) Polyclonal Antibody at dilution of 1:2000)
Western Blot (WB)
(Western Blot analysis of Hela cells using Phospho-Stat5 (Ser726/731) Polyclonal Antibody at dilution of 1:500.)
Western Blot (WB)
(Western Blot analysis of Hela, 293 cells with Phospho-ERK 1/2 (Thr202/Tyr204) Polyclonal Antibody)
Western Blot (WB)
(Western Blot analysis of MCF7 cells with Phospho-Akt (Ser473) Polyclonal Antibody at dilution of 1:1000)
Western Blot (WB)
(Western Blot analysis of 293 cells with Phospho-c-Src (Tyr419) Polyclonal Antibody at dilution of 1:1000)
Western Blot (WB)
(Western Blot analysis of 293 cells with Phospho-PLC gamma1 (Tyr783) Polyclonal Antibody)
Related Product Information for ALK antibody kit
Background: Anaplastic lymphoma kinase (ALK) is a tyrosine kinase receptor for pleiotrophin (PTN), a growth factor involved in embryonic brain development. In ALK-expressing cells, PTN induces phosphorylation of both ALK and the downstream effectors IRS-1, Shc, PLCgamma, and PI3 kinase. ALK was originally discovered as a nucleophosmin (NPM)-ALK fusion protein produced by a translocation. Investigators have found that the NPM-ALK fusion protein is a constitutively active, oncogenic tyrosine kinase associated with anaplastic lymphoma. Research literature suggests that activation of PLCgamma by NPM-ALK may be a crucial step for its mitogenic activity and involved in the pathogenesis of anaplastic lymphomas. A distinct ALK oncogenic fusion protein involving ALK and echinoderm microtubule-associated protein like 4 (EML4) has been described in the research literature from a non-small cell lung cancer (NSCLC) cell line, with corresponding fusion transcripts present in some cases of lung adenocarcinoma. The short, amino-terminal region of the microtubule-associated protein EML4 is fused to the kinase domain of ALK.
