Mouse anti-Human Insulin Monoclonal Antibody | anti-INS antibody

Mouse Monoclonal [clone 2D11.H5] (IgG1,k) to Human Insulin

Cat. Num. AAA245024

• Gene Names: INS; IDDM; ILPR; IRDN; IDDM1; IDDM2; MODY10
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Protein A Purified
• Synonyms: Insulin; Monoclonal Antibody; Mouse Monoclonal [clone 2D11.H5] (IgG1; k) to Human Insulin; Anti-Insulin Antibody (clone 2D11.H5) IHC-plus; IRDN; IDDM2; Preproinsulin; ILPR; MODY10; Proinsulin; Human Insulin; anti-INS antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 2D11.H5
• Specificity: Purified human insulin coupled to bovine serum albumin (BSA). Hybridoma: Produced by the fusion between BALB/c mouse splenocytes and mouse myeloma SP2/0 cells after immunization with insulin from human pancreas using conventional hybridoma technology.
• Purity/Purification: Protein A Purified
• Form/Format: 0.02 M potassium phosphate, 0.5 M sodium chloride, pH 7.2, 0.01% sodium azide.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 110

• Applicable Applications for anti-INS antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA, IHC-P (5 ug/ml), WB; Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...
• Target Species: Human
• Immunogen Description: Fusion protein from human INS / Insulin.
• Immunogen: Insulin antibody was raised against fusion protein from human INS / Insulin.
• Preparation and Storage: +4 degree C or -20 degree C, Avoid repeated freezing and thawing.

Immunohistochemistry (IHC)

(Anti-Insulin antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Dot Blot (DB)

(Anti-Insulin Antibody - Dot Blot. Dot blotting. Mab anti-Insulin antibody (clone 2D11. H5) is shown to detect human insulin by dot blot. Each dot blot represents 1 ul of non-denatured human insulin at various dilutions starting at 1.0 ug/ml spotted on to nitrocellulose. A 1:400 dilution of Mab anti-Insulin is used for 2 hour followed by detection using a 1:5000 dilution of IRDyeTM800 conjugated Goat-a-Mouse IgG [H&L] (reacted for 45 min at room temperature and visualization using the Odyssey Infrared Imaging System developed by LI-COR. Other detection systems will yield similar results. IRDye is a trademark of LI-COR, Inc.)

ELISA (EIA)

(Anti-Insulin Antibody - ELISA. ELISA Results of Mab anti-Insulin antibody tested against human insulin by ELISA. Each well was coated with 0.1ug of conjugate. The starting concentration of antibody in the dilution series was 10 ug/ml. Each point on the Y-axis represents a 3-fold dilution. The midpoint of the titration curve represents approximately 5 ng/ml antibody or a 1:200000 dilution from the stock concentration. HRP conjugated Gt-a-Mouse IgG H&L and TMB substrate were used for detection.)

Related Product Information for anti-INS antibody

Insulin (INS) is a hormone central to regulating carbohydrate and fat metabolism in the body. Insulin causes cells in the liver, muscle, and fat tissue to take up glucose from the blood, storing it as glycogen in the liver and muscle. After removal of the precursor signal peptide, proinsulin is post-translationally cleaved into two chains (peptide A and peptide B) that are covalently linked via two disulfide bonds.

Product Categories/Family for anti-INS antibody

Family: Hormone

NCBI and Uniprot Product Information

• NCBI GI #: 4557671
• NCBI GeneID: 3630
• NCBI Accession #: NP_000198.1
• NCBI GenBank Nucleotide #: NM_000207.2
• UniProt Accession #: P01308; Q5EEX2
• Molecular Weight: 21,537 Da
• NCBI Official Full Name: insulin preproprotein
• NCBI Official Synonym Full Names: insulin
• NCBI Official Symbol: INS
• NCBI Official Synonym Symbols: IDDM; ILPR; IRDN; IDDM1; IDDM2; MODY10
• NCBI Protein Information: insulin; proinsulin; preproinsulin
• UniProt Protein Name: Insulin
• Protein Family: Insulin
• UniProt Gene Name: INS
• UniProt Entry Name: INS_HUMAN

NCBI Description

After removal of the precursor signal peptide, proinsulin is post-translationally cleaved into three peptides: the B chain and A chain peptides, which are covalently linked via two disulfide bonds to form insulin, and C-peptide. Binding of insulin to the insulin receptor (INSR) stimulates glucose uptake. A multitude of mutant alleles with phenotypic effects have been identified. There is a read-through gene, INS-IGF2, which overlaps with this gene at the 5' region and with the IGF2 gene at the 3' region. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2010]

Uniprot Description

Insulin: Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver. Heterodimer of a B chain and an A chain linked by two disulfide bonds. Belongs to the insulin family.

Protein type: Hormone; Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 11p15.5

Cellular Component: extracellular space; endoplasmic reticulum lumen; Golgi lumen; extracellular region

Molecular Function: identical protein binding; insulin-like growth factor receptor binding; protein binding; protease binding; hormone activity; insulin receptor binding

Biological Process: positive regulation of nitric oxide biosynthetic process; glucose homeostasis; positive regulation of vasodilation; activation of NF-kappaB transcription factor; positive regulation of glucose import; negative regulation of protein oligomerization; regulation of protein localization; cell-cell signaling; negative regulation of gluconeogenesis; acute-phase response; regulation of transmembrane transporter activity; negative regulation of NAD(P)H oxidase activity; positive regulation of mitosis; activation of protein kinase B; positive regulation of nitric-oxide synthase activity; negative regulation of acute inflammatory response; glucose metabolic process; positive regulation of lipid biosynthetic process; positive regulation of cell growth; positive regulation of protein amino acid autophosphorylation; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of cell differentiation; regulation of amino acid metabolic process; negative regulation of protein catabolic process; negative regulation of glycogen catabolic process; wound healing; positive regulation of glycogen biosynthetic process; positive regulation of cellular protein metabolic process; glucose transport; regulation of protein secretion; positive regulation of MAPKKK cascade; negative regulation of vasodilation; regulation of transcription, DNA-dependent; negative regulation of lipid catabolic process; positive regulation of cell proliferation; negative regulation of proteolysis; negative regulation of protein secretion; MAPKKK cascade; negative regulation of fatty acid metabolic process; alpha-beta T cell activation; endocrine pancreas development; positive regulation of protein kinase B signaling cascade; G-protein coupled receptor protein signaling pathway; cellular protein metabolic process; fatty acid homeostasis; positive regulation of glycolysis; insulin receptor signaling pathway; energy reserve metabolic process; positive regulation of insulin receptor signaling pathway; regulation of insulin secretion; positive regulation of DNA replication; positive regulation of cytokine secretion; positive regulation of cell migration

Disease: Diabetes Mellitus, Insulin-dependent, 2; Diabetes Mellitus, Permanent Neonatal; Hyperproinsulinemia; Maturity-onset Diabetes Of The Young, Type 10