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Western Blot (WB)
(Western Blot analysis of KB, MCF7 cells using Caspase-3 Polyclonal Antibody at dilution of 1:1000.)
Effector Caspases and Substrates Antibody Kit
Effector Caspases and Substrates Antibody Sampler Kit
Applications
Western Blot
Synonyms
Effector Caspases and Substrates; Effector Caspases and Substrates Antibody Sampler Kit; Effector Caspases and Substrates antibody kit
Applicable Applications for Effector Caspases and Substrates antibody kit
Western Blot (WB)
Western Blot (WB)
(Western Blot analysis of KB, MCF7 cells using Caspase-3 Polyclonal Antibody at dilution of 1:1000.)
Western Blot (WB)
(Western Blot analysis of KB, MCF7 cells using Caspase-3 Polyclonal Antibody at dilution of 1:1000.)
Western Blot (WB)
(Western Blot analysis of HepG2 cells using Caspase-6 Polyclonal Antibody at dilution of 1:1000.)
Western Blot (WB)
(Western Blot analysis of HepG2 cells using Caspase-6 Polyclonal Antibody at dilution of 1:1000.)
Western Blot (WB)
(Western Blot analysis of HT29 cells using Caspase-7 Polyclonal Antibody at dilution of 1:1000.)
Western Blot (WB)
(Western Blot analysis of HT29 cells using Caspase-7 Polyclonal Antibody at dilution of 1:1000.)
Western Blot (WB)
(Western Blot analysis of 3T3 cells using Lamin A/C Polyclonal Antibody at dilution of 1:2000.)
Western Blot (WB)
(Western Blot analysis of 3T3 cells using Lamin A/C Polyclonal Antibody at dilution of 1:2000.)
Western Blot (WB)
(Western Blot analysis of COLO205 cells using Lamin B1 Polyclonal Antibody at dilution of 1:2000.)
Western Blot (WB)
(Western Blot analysis of COLO205 cells using Lamin B1 Polyclonal Antibody at dilution of 1:2000.)
Western Blot (WB)
(Western Blot analysis of Rat musle using PARP-1 Polyclonal Antibody at dilution of 1:1000.)
Western Blot (WB)
(Western Blot analysis of Rat musle using PARP-1 Polyclonal Antibody at dilution of 1:1000.)
Related Product Information for Effector Caspases and Substrates antibody kit
Background: Apoptosis is a regulated physiological process leading to cell death. Caspases, a family of cysteine acid proteases, are central regulators of apoptosis. Caspase-3 (CPP-32, Apoptain, Yama, SCA-1), Caspase-6 (Mch2), and Caspase-7 (CMH-1, Mch3, ICE-LAP3) are effector caspases functioning in cellular apoptotic processes. Upon apoptotic stimulation, initiator caspases such as caspase-9 (ICE-LAP6, Mch6) are cleaved and activated. The activated upstream caspases further process downstream executioner caspases by cleaving them into activated large and small subunits, thereby initiating a caspase cascade leading to apoptosis. PARP, a 116 kDa nuclear poly (ADP-ribose) polymerase, appears to be involved in DNA repair in response to environmental stress. This protein can be cleaved by many ICE-like caspases in vitro and is one of the main cleavage targets of caspase-3 in vivo. In human PARP, cleavage occurs between Asp214 and Gly215, which separates the PARP amino-terminal DNA binding domain (24 kDa) from the carboxy-terminal catalytic domain (89 kDa). PARP helps cells to maintain their viability; cleavage of PARP facilitates cellular disassembly and serves as a marker of cells undergoing apoptosis. Lamins are nuclear membrane structural components that are important in maintaining normal cell functions, such as cell cycle control, DNA replication, and chromatin organization. Lamins have been subdivided into types A and B. Type-A lamins consist of lamin A and C, which arise from alternative splicing of the lamin A gene LMNA. Lamin A and C are cleaved by caspases into large (41-50 kDa) and small (28 kDa) fragments, which can be used as markers for apoptosis. Type-B lamins consist of lamin B1 and B2, encoded by separate genes. Lamin B1 is also cleaved by caspases during apoptosis.
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Product Notes
The Effector Caspases and Substrates (Catalog #AAA2550140) is an Antibody Kit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Effector Caspases and Substrates can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Researchers should empirically determine the suitability of the Effector Caspases and Substrates for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Effector Caspases and Substrates, Antibody Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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