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Western Blot (WB)
(Western Blot analysis of COS7 cells with Phospho-Cdc2 (Tyr15) Polyclonal Antibody)
Cell Cycle Regulation Antibody Kit
Cell Cycle Regulation Antibody Sampler Kit II
Applications
Western Blot
Synonyms
Cell Cycle Regulation; Cell Cycle Regulation Antibody Sampler Kit II; Cell Cycle Regulation antibody kit
Applicable Applications for Cell Cycle Regulation antibody kit
Western Blot (WB)
Western Blot (WB)
(Western Blot analysis of COS7 cells with Phospho-Cdc2 (Tyr15) Polyclonal Antibody)
Western Blot (WB)
(Western Blot analysis of COS7 cells with Phospho-Cdc2 (Tyr15) Polyclonal Antibody)
Western Blot (WB)
(Western Blot analysis of HEK-293 cells using Cyclin A2 Monoclonal Antibody at dilution of 1:4000)
Western Blot (WB)
(Western Blot analysis of HEK-293 cells using Cyclin A2 Monoclonal Antibody at dilution of 1:4000)
Western Blot (WB)
(Western Blot analysis of HepG2 cells using Cyclin B1 Polyclonal Antibody at dilution of 1:500.)
Western Blot (WB)
(Western Blot analysis of HepG2 cells using Cyclin B1 Polyclonal Antibody at dilution of 1:500.)
Western Blot (WB)
(Western Blot analysis of KB cells with Phospho-Histone H3 (Ser10) Polyclonal Antibody)
Western Blot (WB)
(Western Blot analysis of KB cells with Phospho-Histone H3 (Ser10) Polyclonal Antibody)
Western Blot (WB)
(Western Blot analysis of Hela cells using Myt 1 Polyclonal Antibody at dilution of 1:2000.)
Western Blot (WB)
(Western Blot analysis of Hela cells using Myt 1 Polyclonal Antibody at dilution of 1:2000.)
Western Blot (WB)
(Western Blot analysis of COLO205 cells with p21 Polyclonal Antibody.)
Western Blot (WB)
(Western Blot analysis of COLO205 cells with p21 Polyclonal Antibody.)
Western Blot (WB)
(Western Blot analysis of 293 cells with Phospho-Wee1 (Ser642) Polyclonal Antibody.)
Western Blot (WB)
(Western Blot analysis of 293 cells with Phospho-Wee1 (Ser642) Polyclonal Antibody.)
Related Product Information for Cell Cycle Regulation antibody kit
Background: The critical regulatory step in activating cdc2 during progression into mitosis appears to be dephosphorylation of cdc2 at Tyr15 and Thr14. Phosphorylation of cdc2 by the protein kinases Wee1 and Myt1 at Thr14 and Tyr15 results in inhibition of cdc2. Progression through the G1/S checkpoint and initiation of DNA replication requires cyclin E; traversing the G2/M checkpoint to initiate mitosis requires cyclin B, and cyclin A may be required for both S-phase and M-phase. The versatile p21 cyclin- dependent kinase inhibitor, which interacts with several cyclin-CDK complexes to regulate cyclin-CDK during the cell cycle, is regulated by phosphorylation and ubiquitin-mediated degradation. Phosphorylation of histone H3 at Ser10 and neighboring residues correlates with chromosomal condensation, which is essential for segregation of chromosomes during mitosis.
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Product Notes
The Cell Cycle Regulation (Catalog #AAA2550130) is an Antibody Kit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Cell Cycle Regulation can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Researchers should empirically determine the suitability of the Cell Cycle Regulation for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Cell Cycle Regulation, Antibody Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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