Human SIGLEC10 ELISA Kit | SIGLEC10 elisa kit

Human SIGLEC10 (Sialic Acid Binding Immunoglobulin Like Lectin 10) ELISA Kit

Cat. Num. AAA2509371

• Gene Names: SIGLEC10; SLG2; PRO940; SIGLEC-10
• Reactivity: Human
• Synonyms: SIGLEC10; Human SIGLEC10 (Sialic Acid Binding Immunoglobulin Like Lectin 10) ELISA Kit; SIGLEC10 elisa kit

• Reactivity: Human
• Specificity: This kit recognizes Human SIGLEC10 in samples. No significant cross-reactivity or interference between Human SIGLEC10 and analogues was observed.
• Sequence Length: 454

• Samples: Serum, plasma and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.16-10ng/mL
• Sensitivity: 0.1ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human SIGLEC10 were tested 20 times on one plate, respectively.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human SIGLEC10 were tested on 3 different plates, 20 replicates in each plate.
• Preparation and Storage: Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SIGLEC10 elisa kit

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human SIGLEC10 concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human SIGLEC10. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human SIGLEC10 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human SIGLEC10, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human SIGLEC10. You can calculate the concentration of Human SIGLEC10 in the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 283837888
• NCBI GeneID: 89790
• NCBI Accession #: NP_001164632.1
• NCBI GenBank Nucleotide #: NM_001171161.1
• UniProt Accession #: Q96LC7; Q3MIR5; Q6UXI8; Q96G54; Q96LC8; A8K1I5; A8K3C7; C9JJ33; C9JM10; F8W917
• Molecular Weight: 57,486 Da
• NCBI Official Full Name: sialic acid-binding Ig-like lectin 10 isoform 7
• NCBI Official Synonym Full Names: sialic acid binding Ig-like lectin 10
• NCBI Official Symbol: SIGLEC10
• NCBI Official Synonym Symbols: SLG2; PRO940; SIGLEC-10
• NCBI Protein Information: sialic acid-binding Ig-like lectin 10; sialic acid binding Ig-like lectin 10 Ig-like lectin 7; siglec-like gene 2; siglec-like protein 2
• UniProt Protein Name: Sialic acid-binding Ig-like lectin 10
• Protein Family: Sialic acid-binding Ig-like lectin
• UniProt Gene Name: SIGLEC10
• UniProt Synonym Gene Names: SLG2; Siglec-10
• UniProt Entry Name: SIG10_HUMAN

NCBI Description

SIGLECs are members of the immunoglobulin superfamily that are expressed on the cell surface. Most SIGLECs have 1 or more cytoplasmic immune receptor tyrosine-based inhibitory motifs, or ITIMs. SIGLECs are typically expressed on cells of the innate immune system, with the exception of the B-cell expressed SIGLEC6 (MIM 604405).[supplied by OMIM, Jul 2002]

Uniprot Description

Siglec-10: a cell surface protein belonging to the immunoglobulin superfamily and the SIGLEC (sialic acid binding Ig-like lectin) family. Most SIGLECs have 1 or more cytoplasmic immune receptor tyrosine-based inhibitory motifs, or ITIMs. Putative adhesion molecule that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha2,3- or 2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. SIGLECs are typically expressed on cells of the innate immune system, with the exception of the B-cell expressed SIGLEC6. In the immune response, may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. Five alternatively spliced isoforms have been described.

Protein type: Cell adhesion; Membrane protein, integral

Chromosomal Location of Human Ortholog: 19q13.3

Cellular Component: integral to membrane; plasma membrane; extracellular region

Molecular Function: carbohydrate binding

Biological Process: cell adhesion