Mouse CDK1 / CDC2 Monoclonal Antibody | anti-CDK1 antibody

Mouse Monoclonal [clone POH-1] (IgG2a) to Human CDK1 / CDC2

Cat. Num. AAA246344

• Gene Names: CDK1; CDC2; CDC28A; P34CDC2
• Reactivity: Bovine, Human, Primate
• Applications: Immunohistochemistry, Immunocytochemistry, Western Blot, Immunoprecipitation
• Purity: Protein A Purified
• Synonyms: CDK1 / CDC2; Monoclonal Antibody; Mouse Monoclonal [clone POH-1] (IgG2a) to Human CDK1 / CDC2; Anti-CDK1 / CDC2 Antibody (clone POH-1) IHC-plus; CDK1; CDC28A; CDC2; CDKN1; Cyclin-dependent kinase 1; p34 protein kinase; p34CDC2; CDC2a; Cell cycle controller CDC2; Cell division protein kinase 1; PSTAIR; Human CDK1; anti-CDK1 antibody

• Host: Mouse
• Reactivity: Bovine, Human, Primate
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone Number: POH-1
• Specificity: The antibody POH-1 specifically detects Cdk1 (p34Cdc2). Staining of Cdk1 reflects the proliferating potential of respective tissue.
• Purity/Purification: Protein A Purified
• Form/Format: PBS, 15 mM sodium azide, approx., pH 7.4.
• Sequence Length: 297

• Applicable Applications for anti-CDK1 antibody: Immunohistochemistry (IHC - Paraffin), Immunocytochemistry (ICC), Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: ICC, IHC-P (10 ug/ml), IP, WB; Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...
• Target Species: Human
• Non Reactive Species: Drosophila, Xenopus, Mouse, Rat
• Immunogen Description: Recombinant human cdc2 protein.
• Immunogen Type: Recombinant protein
• Immunogen: CDK1 / CDC2 antibody was raised against recombinant human cdc2 protein.
• Preparation and Storage: Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles.

Immunohistochemistry (IHC)

(Anti-Cdc2 antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

Immunohistochemistry (IHC)

(Anti-Cdc2 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

Related Product Information for anti-CDK1 antibody

CDC2/CDK1, a CDC2/CDK -type protein kinase, regulates cell cycle transitions G1 to S and G2 to M. CDC2/CDK1 is maintained in an inactive state by phosphorylation by kinases MYT1 and WEE1. The active kinase, called the M-phase promoting factor, consists of a heterodimer with cyclin B, and final activation at G2/M results from CDC25 phosphatase-mediated dephosphorylation of CDC2/CDK1.

Product Categories/Family for anti-CDK1 antibody

Family: Protein Kinase; Subfamily: CDC2/CDK

NCBI and Uniprot Product Information

• NCBI GI #: 4502709
• NCBI GeneID: 983
• NCBI Accession #: NP_001777.1
• NCBI GenBank Nucleotide #: NM_001786.4
• UniProt Accession #: P06493; O60764; A8K7C4; C9J497
• Molecular Weight: 27,503 Da
• NCBI Official Full Name: cyclin-dependent kinase 1 isoform 1
• NCBI Official Synonym Full Names: cyclin-dependent kinase 1
• NCBI Official Symbol: CDK1
• NCBI Official Synonym Symbols: CDC2; CDC28A; P34CDC2
• NCBI Protein Information: cyclin-dependent kinase 1; p34 protein kinase; cell cycle controller CDC2; cell division protein kinase 1; cell division control protein 2 homolog; cell division cycle 2, G1 to S and G2 to M
• UniProt Protein Name: Cyclin-dependent kinase 1
• UniProt Gene Name: CDK1
• UniProt Synonym Gene Names: CDC2; CDC28A; CDKN1; P34CDC2; CDK1
• UniProt Entry Name: CDK1_HUMAN

NCBI Description

The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with this protein and function as regulatory subunits. The kinase activity of this protein is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of this protein also play important regulatory roles in cell cycle control. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]

Uniprot Description

CDK1: a protein kinase of the CDK family. Catalytic subunit of the conserved protein complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions. Mitotic cyclins stably associate with this protein and function as regulatory subunits. Its activity is controlled by cyclin availability and phosphorylation through the cell cycle. Activated in many cancers including colon, liver and breast. The T isoform, which lacks a regulatory region, is expressed in breast cancer. Inhibition in cancer cells may drive cells into apoptosis. May also drive cell migration. Inhibitors: BMS-265246, BMS-265246-01. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Protein kinase, Ser/Thr (non-receptor); Protein kinase, CMGC; EC 2.7.11.23; Motility/polarity/chemotaxis; EC 2.7.11.22; Kinase, protein; Cell cycle regulation; CMGC group; CDK family; CDK1 subfamily; CDK/CDK1 subfamily

Chromosomal Location of Human Ortholog: 10q21.1

Cellular Component: nucleoplasm; centrosome; membrane; mitochondrion; spindle microtubule; cytoplasm; midbody; nucleus; cytosol

Molecular Function: RNA polymerase subunit kinase activity; protein serine/threonine kinase activity; protein binding; cyclin binding; cyclin-dependent protein kinase activity; histone kinase activity; Hsp70 protein binding; ATP binding; protein kinase activity

Biological Process: regulation of Schwann cell differentiation; activation of MAPKK activity; nerve growth factor receptor signaling pathway; activation of MAPK activity; histone phosphorylation; response to toxin; ventricular cardiac muscle cell development; regulation of embryonic development; stress-activated MAPK cascade; centrosome cycle; toll-like receptor 3 signaling pathway; response to organic cyclic substance; toll-like receptor 5 signaling pathway; small GTPase mediated signal transduction; protein complex assembly; G2/M transition of mitotic cell cycle; toll-like receptor 4 signaling pathway; positive regulation of cardiac muscle cell proliferation; response to drug; mitosis; organ regeneration; fibroblast growth factor receptor signaling pathway; positive regulation of protein import into nucleus, translocation; cell aging; pronuclear fusion; toll-like receptor 2 signaling pathway; chromosome condensation; response to ethanol; cell division; response to activity; toll-like receptor 9 signaling pathway; response to amine stimulus; G1/S transition of mitotic cell cycle; negative regulation of apoptosis; positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; axon guidance; apoptosis; mitotic nuclear envelope disassembly; positive regulation of mitotic cell cycle; toll-like receptor 10 signaling pathway; epithelial cell differentiation; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; response to axon injury; DNA replication; epidermal growth factor receptor signaling pathway; cell migration; MyD88-independent toll-like receptor signaling pathway; organelle organization and biogenesis; MAPKKK cascade; peptidyl-threonine phosphorylation; microtubule cytoskeleton organization and biogenesis; DNA repair; G1/S-specific transcription in mitotic cell cycle; MyD88-dependent toll-like receptor signaling pathway; peptidyl-serine phosphorylation; response to cadmium ion; response to copper ion; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; mitotic cell cycle G2/M transition DNA damage checkpoint; Ras protein signal transduction; toll-like receptor signaling pathway; insulin receptor signaling pathway; innate immune response; mitotic cell cycle; vascular endothelial growth factor receptor signaling pathway; positive regulation of DNA replication