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Typical Testing Data/Standard Curve (for reference only)

Mouse angiopoietin 2 ELISA Kit | ANGPT2 elisa kit

Mouse Angiopoietin 2, ANG-2 ELISA Kit

Gene Names
Angpt2; Ang2; Agpt2; Ang-2
Reactivity
Mouse
Synonyms
angiopoietin 2; Mouse Angiopoietin 2; ANG-2 ELISA Kit; AGPT2; ANG2; Tie2-ligand; angiopoietin-2; angiopoietin-2B; angiopoietin-2a; ANGPT2 elisa kit
Ordering
For Research Use Only!
Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of mouse ANG-2. No significant cross-reactivity or interference between mouse ANG-2 and analogues was observed.
Sequence Length
496
Samples
Serum, plasma, tissue homogenates, cell culture supernates
Assay Type
Quantitative Sandwich
Detection Range
0.156 pg/ml-10 pg/ml
Sensitivity
< 0.039 pg/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Typical Testing Data/Standard Curve (for reference only)
Related Product Information for ANGPT2 elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ANG-2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ANG-2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ANG-2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ANG-2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
56,576 Da
NCBI Official Full Name
angiopoietin-2
NCBI Official Synonym Full Names
angiopoietin 2
NCBI Official Symbol
Angpt2
NCBI Official Synonym Symbols
Ang2; Agpt2; Ang-2
NCBI Protein Information
angiopoietin-2
UniProt Protein Name
Angiopoietin-2
Protein Family
UniProt Gene Name
Angpt2
UniProt Synonym Gene Names
Agpt2; ANG-2
UniProt Entry Name
ANGP2_MOUSE

NCBI Description

This gene encodes an endothelial cell (EC)-derived regulator of angiogenesis and ligand for endothelial-specific receptor tyrosine kinase. The encoded protein acts as an anti-apoptotic factor for stressed ECs and a proapoptotic factor for resting ECs. [provided by RefSeq, Jan 2013]

Uniprot Description

Function: Binds to TEK/TIE2, competing for the ANGPT1 binding site, and modulating ANGPT1 signaling. Can induce tyrosine phosphorylation of TEK/TIE2 in the absence of ANGPT1. In the absence of angiogenic inducers, such as VEGF, ANGPT2-mediated loosening of cell-matrix contacts may induce endothelial cell apoptosis with consequent vascular regression. In concert with VEGF, it may facilitate endothelial cell migration and proliferation, thus serving as a permissive angiogenic signal

Research Articles on ANGPT2

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Product Notes

The Mouse ANGPT2 angpt2 (Catalog #AAA702253) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA702253 ELISA Kit recognizes Mouse ANGPT2. It is sometimes possible for the material contained within the vial of "angiopoietin 2, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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