Mouse Angiopoietin 2 (ANGPT2) RTU ELISA Kit | ANGPT2 rtu elisa kit

Mouse Angiopoietin 2 (ANGPT2) ELISA Kit

Cat. Num. AAA457610

• Gene Names: Angpt2; Ang2; Agpt2; Ang-2
• Reactivity: Mouse
• Synonyms: Angiopoietin 2 (ANGPT2); Mouse Angiopoietin 2 (ANGPT2) ELISA Kit; ANG2; AGPT2; ANGPT2 rtu elisa kit

• Reactivity: Mouse
• Sequence Length: 496

• Assay Type: Sandwich
• Samples: Serum, Plasma, Tissue Homogenates or Other Biological Fluids.
• Detection Range: 15.6-1000pg/mL
• Sensitivity: 6.1pg/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ANGPT2 rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to ANGPT2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to ANGPT2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ANGPT2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of ANGPT2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 31982508
• NCBI GeneID: 11601
• NCBI Accession #: NP_031452.2
• NCBI GenBank Nucleotide #: NM_007426.4
• UniProt Accession #: O35608; Q3U1A1; Q9D2D2
• Molecular Weight: 56,576 Da
• NCBI Official Full Name: angiopoietin-2
• NCBI Official Synonym Full Names: angiopoietin 2
• NCBI Official Symbol: Angpt2
• NCBI Official Synonym Symbols: Ang2; Agpt2; Ang-2
• NCBI Protein Information: angiopoietin-2
• UniProt Protein Name: Angiopoietin-2
• Protein Family: Angiopoietin
• UniProt Gene Name: Angpt2
• UniProt Synonym Gene Names: Agpt2; ANG-2

NCBI Description

This gene encodes an endothelial cell (EC)-derived regulator of angiogenesis and ligand for endothelial-specific receptor tyrosine kinase. The encoded protein acts as an anti-apoptotic factor for stressed ECs and a proapoptotic factor for resting ECs. [provided by RefSeq, Jan 2013]

Uniprot Description

Binds to TEK/TIE2, competing for the ANGPT1 binding site, and modulating ANGPT1 signaling. Can induce tyrosine phosphorylation of TEK/TIE2 in the absence of ANGPT1. In the absence of angiogenic inducers, such as VEGF, ANGPT2-mediated loosening of cell-matrix contacts may induce endothelial cell apoptosis with consequent vascular regression. In concert with VEGF, it may facilitate endothelial cell migration and proliferation, thus serving as a permissive angiogenic signal ().