Western Blot (WB)
(Mouse Ern1 Antibody (C-term) western blot analysis in mouse liver tissue lysates (35ug/lane).This demonstrates the Ern1 antibody detected the Ern1 protein (arrow).)
Rabbit anti-Mouse Ern1 Polyclonal Antibody | anti-Ern1 antibody
Ern1, CT (Ern1, Ire1, Serine/threonine-protein kinase/endoribonuclease IRE1, Endoplasmic reticulum-to-nucleus signaling 1, Inositol-requiring protein 1, Ire1-alpha, Serine/threonine-protein kinase, Endoribonuclease) (Biotin)
Gene Names
Ern1; Ire1a; Ire1p; C85377; AI225830; Ire1alpha; 9030414B18Rik
Reactivity
Mouse
Applications
Western Blot, ELISA
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
Ern1; Polyclonal Antibody; CT (Ern1; Ire1; Serine/threonine-protein kinase/endoribonuclease IRE1; Endoplasmic reticulum-to-nucleus signaling 1; Inositol-requiring protein 1; Ire1-alpha; Serine/threonine-protein kinase; Endoribonuclease) (Biotin); Endoribonuclease; anti-Ern1 antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Applicable Applications for anti-Ern1 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:100-500
ELISA: 1:1,000
Applications are based on unconjugated antibody.
ELISA: 1:1,000
Applications are based on unconjugated antibody.
Immunogen
Mouse Ern1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 826-855 amino acids from the C-terminal region of Mouse Ern1.
Conjugate
Biotin
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Mouse Ern1 Antibody (C-term) western blot analysis in mouse liver tissue lysates (35ug/lane).This demonstrates the Ern1 antibody detected the Ern1 protein (arrow).)
Western Blot (WB)
(Mouse Ern1 Antibody (C-term) western blot analysis in mouse liver tissue lysates (35ug/lane).This demonstrates the Ern1 antibody detected the Ern1 protein (arrow).)
Related Product Information for anti-Ern1 antibody
Ern1 senses unfolded proteins in the lumen of the endoplasmic reticulum via its N-terminal domain which leads to enzyme auto-activation. The active endoribonuclease domain splices XBP1 mRNA to generate a new C-terminus, converting it into a potent unfolded-protein response transcriptional activator and triggering growth arrest and apoptosis.
Product Categories/Family for anti-Ern1 antibody
References
Li, H., et al. Proc. Natl. Acad. Sci. U.S.A. 107(37):16113-16118(2010). Auf, G., et al. Proc. Natl. Acad. Sci. U.S.A. 107(35):15553-15558(2010). Oikawa, D., et al. FEBS Lett. 584(5):1066-1070(2010). Yang, L., et al. PLoS ONE 5 (7), E11621 (2010): Ghosh, R., et al. PLoS ONE 5 (3), E9575 (2010).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
110,185 Da
NCBI Official Full Name
serine/threonine-protein kinase/endoribonuclease IRE1
NCBI Official Synonym Full Names
endoplasmic reticulum (ER) to nucleus signalling 1
NCBI Official Symbol
Ern1
NCBI Official Synonym Symbols
Ire1a; Ire1p; C85377; AI225830; Ire1alpha; 9030414B18Rik
NCBI Protein Information
serine/threonine-protein kinase/endoribonuclease IRE1; ire1-alpha; inositol-requiring 1 alpha; inositol-requiring protein 1; endoplasmic reticulum-to-nucleus signaling 1
UniProt Protein Name
Serine/threonine-protein kinase/endoribonuclease IRE1
UniProt Gene Name
Ern1
UniProt Synonym Gene Names
Ire1; IRE1a
UniProt Entry Name
ERN1_MOUSE
Similar Products
Product Notes
The Ern1 ern1 (Catalog #AAA6296604) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Ern1, CT (Ern1, Ire1, Serine/threonine-protein kinase/endoribonuclease IRE1, Endoplasmic reticulum-to-nucleus signaling 1, Inositol-requiring protein 1, Ire1-alpha, Serine/threonine-protein kinase, Endoribonuclease) (Biotin) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Ern1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:100-500 ELISA: 1:1,000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the Ern1 ern1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Ern1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
