ANGPT1 recombinant protein

ANGPT1, 20-498aa, Human, E Coli (Denatured)

Cat. Num. AAA204333

• Gene Names: ANGPT1; AGP1; AGPT; ANG1
• Applications: SDS-Page
• Purity: > 85% by SDS-PAGE
• Synonyms: ANGPT1; 20-498aa; Human; E Coli (Denatured); Angiopoietin 1; AGP1; AGPT; ANG1; ANGPT1 recombinant protein

• Host: E Coli
• Purity/Purification: > 85% by SDS-PAGE
• Form/Format: Liquid. In 20mM Tris-HCl Buffer (pH 8.0) 1M urea, 5% glycerol.
• Concentration: 1 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MSNQRRSPEN SGRRYNRIQH GQCAYTFILP EHDGNCREST TDQYNTNALQ RDAPHVEPDF SSQKLQHLEH VMENYTQWLQ KLENYIVENM KSEMAQIQQN AVQNHTATML EIGTSLLSQT AEQTRKLTDV ETQVLNQTSR LEIQLLENSL STYKLEKQLL QQTNEILKIH EKNSLLEHKI LEMEGKHKEE LDTLKEEKEN LQGLVTRQTY IIQELEKQLN RATTNNSVLQ KQQLELMDTV HNLVNLCTKE GVLLKGGKRE EEKPFRDCAD VYQAGFNKSG IYTIYINNMP EPKKVFCNMD VNGGGWTVIQ HREDGSLDFQ RGWKEYKMGF GNPSGEYWLG NEFIFAITSQ RQYMLRIELM DWEGNRAYSQ YDRFHIGNEK QNYRLYLKGH TGTAGKQSSL ILHGADFSTK DADNDNCMCK CALMLTGGWW FDACGPSNLN GMFYTAGQNH GKLNGIKWHY FKGPSYSLRS TTMMIRPLDF
• Sequence Length: 498

• Applicable Applications for ANGPT1 recombinant protein: SDS-PAGE
• Antigen Species: Human
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for ANGPT1 recombinant protein

Human ANGPT1 is a secreted ligand for Tie-2, a cell surface receptor tyrosine kinase expressed in endothelial and hemopoietic cells. This protein is glycosylated and has a fibrinogen-like domain at the carboxy terminus and coiled-coil regions in the amino terminus. ANGPT1 is an angiogenic factor that mediates blood vessel maturation and may be involved in endothelial development. Recombinant human ANGPT1 protein was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for ANGPT1 recombinant protein

Apoptosis

References

Kopczynska E., et al. (2009) Eur J Gynaecol Oncol. 30 (6):646-9.; Davis S., et al. (1996) Cell. 87 (7):1161-9.

NCBI and Uniprot Product Information

• NCBI GI #: 20532340
• NCBI GeneID: 284
• NCBI Accession #: NP_001137
• NCBI GenBank Nucleotide #: NM_001146.4
• UniProt Accession #: Q15389; Q5HYA0
• Molecular Weight: 55.6 kDa (480aa)
• NCBI Official Full Name: angiopoietin-1 isoform 1
• NCBI Official Synonym Full Names: angiopoietin 1
• NCBI Official Symbol: ANGPT1
• NCBI Official Synonym Symbols: AGP1; AGPT; ANG1
• NCBI Protein Information: angiopoietin-1
• UniProt Protein Name: Angiopoietin-1
• Protein Family: Angiopoietin
• UniProt Gene Name: ANGPT1
• UniProt Synonym Gene Names: KIAA0003; ANG-1

NCBI Description

This gene encodes a secreted glycoprotein that belongs to the angiopoietin family. Members of this family play important roles in vascular development and angiogenesis. All angiopoietins bind with similar affinity to an endothelial cell-specific tyrosine-protein kinase receptor. The protein encoded by this gene is a secreted glycoprotein that activates the receptor by inducing its tyrosine phosphorylation. It plays a critical role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme and inhibits endothelial permeability. The protein also contributes to blood vessel maturation and stability, and may be involved in early development of the heart. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Sep 2015]

Uniprot Description

Binds and activates TEK/TIE2 receptor by inducing its dimerization and tyrosine phosphorylation. Plays an important role in the regulation of angiogenesis, endothelial cell survival, proliferation, migration, adhesion and cell spreading, reorganization of the actin cytoskeleton, but also maintenance of vascular quiescence. Required for normal angiogenesis and heart development during embryogenesis. After birth, activates or inhibits angiogenesis, depending on the context. Inhibits angiogenesis and promotes vascular stability in quiescent vessels, where endothelial cells have tight contacts. In quiescent vessels, ANGPT1 oligomers recruit TEK to cell-cell contacts, forming complexes with TEK molecules from adjoining cells, and this leads to preferential activation of phosphatidylinositol 3-kinase and the AKT1 signaling cascades. In migrating endothelial cells that lack cell-cell adhesions, ANGT1 recruits TEK to contacts with the extracellular matrix, leading to the formation of focal adhesion complexes, activation of PTK2/FAK and of the downstream kinases MAPK1/ERK2 and MAPK3/ERK1, and ultimately to the stimulation of sprouting angiogenesis. Mediates blood vessel maturation/stability. Implicated in endothelial developmental processes later and distinct from that of VEGF. Appears to play a crucial role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme.