Porcine ANG1 (Angiopoietin-1) ELISA Kit | ANG1 elisa kit

Porcine ANG1 (Angiopoietin-1) ELISA Kit

Cat. Num. AAA7606733

• Gene Names: ANGPT1; AGP1; AGPT; ANG1
• Reactivity: Porcine
• Synonyms: ANG1 (Angiopoietin-1); Porcine ANG1 (Angiopoietin-1) ELISA Kit; Angiopoietin-1; ANG1 elisa kit

• Reactivity: Porcine
• Sequence Length: 498

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 78.125-5000pg/ml
• Sensitivity: 46.875pg/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ANG1 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- ANG1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-ANG1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the ANG1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of ANG1 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 20532340
• NCBI GeneID: 284
• NCBI Accession #: NP_001137.2
• NCBI GenBank Nucleotide #: NM_001146.4
• UniProt Accession #: Q15389; Q5HYA0
• Molecular Weight: 57,456 Da
• NCBI Official Full Name: angiopoietin-1 isoform 1
• NCBI Official Synonym Full Names: angiopoietin 1
• NCBI Official Symbol: ANGPT1
• NCBI Official Synonym Symbols: AGP1; AGPT; ANG1
• NCBI Protein Information: angiopoietin-1
• UniProt Protein Name: Angiopoietin-1
• Protein Family: Angiogenin
• UniProt Gene Name: ANGPT1
• UniProt Synonym Gene Names: KIAA0003; ANG-1

NCBI Description

This gene encodes a secreted glycoprotein that belongs to the angiopoietin family. Members of this family play important roles in vascular development and angiogenesis. All angiopoietins bind with similar affinity to an endothelial cell-specific tyrosine-protein kinase receptor. The protein encoded by this gene is a secreted glycoprotein that activates the receptor by inducing its tyrosine phosphorylation. It plays a critical role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme and inhibits endothelial permeability. The protein also contributes to blood vessel maturation and stability, and may be involved in early development of the heart. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Sep 2015]

Uniprot Description

ANGPT1: Binds and activates TEK/TIE2 receptor by inducing its dimerization and tyrosine phosphorylation. Plays an important role in the regulation of angiogenesis, endothelial cell survival, proliferation, migration, adhesion and cell spreading, reorganization of the actin cytoskeleton, but also maintenance of vascular quiescence. Required for normal angiogenesis and heart development during embryogenesis. After birth, activates or inhibits angiogenesis, depending on the context. Inhibits angiogenesis and promotes vascular stability in quiescent vessels, where endothelial cells have tight contacts. In quiescent vessels, ANGPT1 oligomers recruit TEK to cell-cell contacts, forming complexes with TEK molecules from adjoining cells, and this leads to preferential activation of phosphatidylinositol 3-kinase and the AKT1 signaling cascades. In migrating endothelial cells that lack cell-cell adhesions, ANGT1 recruits TEK to contacts with the extracellular matrix, leading to the formation of focal adhesion complexes, activation of PTK2/FAK and of the downstream kinases MAPK1/ERK2 and MAPK3/ERK1, and ultimately to the stimulation of sprouting angiogenesis. Mediates blood vessel maturation/stability. Implicated in endothelial developmental processes later and distinct from that of VEGF. Appears to play a crucial role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 8q23.1

Cellular Component: extracellular region; extracellular space; lipid raft; microvillus; plasma membrane

Molecular Function: Ras guanyl-nucleotide exchange factor activity; receptor tyrosine kinase binding

Biological Process: heparin biosynthetic process; leukocyte migration; MAPKKK cascade; negative regulation of apoptosis; negative regulation of cell adhesion; negative regulation of vascular permeability; positive chemotaxis; positive regulation of blood vessel endothelial cell migration; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of protein kinase B signaling cascade; positive regulation of protein ubiquitination; positive regulation of receptor internalization; regulation of endothelial cell proliferation; regulation of satellite cell proliferation; sprouting angiogenesis; Tie receptor signaling pathway; transmembrane receptor protein tyrosine kinase activation (dimerization)