Western Blot (WB)
(Western Blot analysis in mouse brain (Lane 1), human brain (Lane 2) and rat brain (Lane 3) were resolved by electrophoresis, transferred to PVDF membrane and probed with MBS618780 (1:1000).)
Rabbit Dopamine Receptor, D2L, D2S Polyclonal Antibody | anti-DRD2 antibody
Dopamine Receptor, D2L, D2S
Purified by immunoaffinity chromotography.
Purified by immunoaffinity chromotography.
Dilution: ELISA: 0.5-1.0ug/ml
Western Blot: 1:1000. Detected Dopamine Receptor D2 on 10ug of human brain lysates.
Immunoprecipitation: 1-10ug/ml. Use 100-400ug of protein. An antibody made to this epitope has been shown to immunoprecipitate both the long and short forms.
Immunohistochemistry (Paraformaldehyde fixed sections): 2-10ug/ml
Western Blot (WB)
(Western Blot analysis in mouse brain (Lane 1), human brain (Lane 2) and rat brain (Lane 3) were resolved by electrophoresis, transferred to PVDF membrane and probed with MBS618780 (1:1000).)
Western Blot (WB)
(Western Blot analysis in mouse brain (Lane 1), human brain (Lane 2) and rat brain (Lane 3) were resolved by electrophoresis, transferred to PVDF membrane and probed with MBS618780 (1:1000).)
Western Blot (WB)
(Western Blot Analysis: Human brain lysate was resolved by electrophoresis, transferred to PVDF membrane and probed with MBS618780 (1:1000).)
Western Blot (WB)
(Western Blot Analysis: Human brain lysate was resolved by electrophoresis, transferred to PVDF membrane and probed with MBS618780 (1:1000).)
Testing Data
(Staining of the D2 receptor in mouse brain striatum.)
Testing Data
(Staining of the D2 receptor in mouse brain striatum.)
Immunohistochemistry (IHC)
(Immunohistochemistry staining of the D2 Receptor using MBS618780 and of the D4 receptor (using a D4 antibody) in the eutorhiral cortex of monkey brain tissue.)
Immunohistochemistry (IHC)
(Immunohistochemistry staining of the D2 Receptor using MBS618780 and of the D4 receptor (using a D4 antibody) in the eutorhiral cortex of monkey brain tissue.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes the D2 subtype of the dopamine receptor. This G-protein coupled receptor inhibits adenylyl cyclase activity. A missense mutation in this gene causes myoclonus dystonia; other mutations have been associated with schizophrenia. Alternative splicing of this gene results in two transcript variants encoding different isoforms. A third variant has been described, but it has not been determined whether this form is normal or due to aberrant splicing. [provided by RefSeq, Jul 2008]
Uniprot Description
DRD2: Dopamine receptor whose activity is mediated by G proteins which inhibit adenylyl cyclase. Defects in DRD2 are associated with dystonia type 11 (DYT11); also known as alcohol-responsive dystonia. DYT11 is a myoclonic dystonia. Dystonia is defined by the presence of sustained involuntary muscle contractions, often leading to abnormal postures. DYT11 is characterized by involuntary lightning jerks and dystonic movements and postures alleviated by alcohol. Inheritance is autosomal dominant. The age of onset, pattern of body involvement, presence of myoclonus and response to alcohol are all variable. Belongs to the G-protein coupled receptor 1 family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral; GPCR, family 1; Membrane protein, multi-pass; Receptor, GPCR
Chromosomal Location of Human Ortholog: 11q23
Cellular Component: synaptic vesicle membrane; endocytic vesicle; axon; integral to plasma membrane; dendrite; postsynaptic density; dendritic spine; plasma membrane; acrosome; perikaryon; nerve terminal; lateral plasma membrane
Molecular Function: dopamine D2 receptor-like receptor activity; ionotropic glutamate receptor binding; identical protein binding; protein binding; potassium channel regulator activity; protein homodimerization activity; protein heterodimerization activity; dopamine binding; drug binding
Biological Process: response to nicotine; elevation of cytosolic calcium ion concentration during G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); prepulse inhibition; positive regulation of dopamine uptake; response to toxin; positive regulation of receptor internalization; regulation of phosphoprotein phosphatase activity; thermoregulation; positive regulation of multicellular organism growth; dopamine metabolic process; adult walking behavior; dopamine receptor, adenylate cyclase inhibiting pathway; negative regulation of insulin secretion; protein localization; negative regulation of blood pressure; phosphatidylinositol metabolic process; response to drug; response to inactivity; response to light stimulus; positive regulation of neuroblast proliferation; cerebral cortex GABAergic interneuron migration; negative regulation of synaptic transmission, glutamatergic; regulation of sodium ion transport; arachidonic acid secretion; regulation of synaptic transmission, GABAergic; positive regulation of growth hormone secretion; G-protein coupled receptor internalization; reduction of cytosolic calcium ion concentration; activation of protein kinase activity; positive regulation of transcription from RNA polymerase II promoter; regulation of long-term neuronal synaptic plasticity; synaptic transmission, dopaminergic; peristalsis; branching morphogenesis of a nerve; negative regulation of circadian sleep/wake cycle, sleep; response to morphine; locomotory behavior; behavioral response to ethanol; orbitofrontal cortex development; negative regulation of cell proliferation; synaptogenesis; behavioral response to cocaine; visual learning; adenohypophysis development; nerve-nerve synaptic transmission; feeding behavior; circadian regulation of gene expression; response to axon injury; negative regulation of dopamine secretion; response to iron ion; negative regulation of cell migration; associative learning; positive regulation of cytokinesis; grooming behavior; negative regulation of protein secretion; negative regulation of protein kinase B signaling cascade; Wnt receptor signaling pathway; striatum development; regulation of cAMP metabolic process; regulation of heart rate; regulation of potassium ion transport; sensory perception of smell; negative regulation of adenylate cyclase activity; response to amphetamine; auditory behavior; regulation of dopamine uptake; response to cocaine; cellular calcium ion homeostasis; regulation of systemic arterial blood pressure by neurological process; negative regulation of dopamine receptor signaling pathway; pigmentation; long-term memory; axonogenesis; release of sequestered calcium ion into cytosol; positive regulation of G-protein coupled receptor protein signaling pathway; response to hypoxia; regulation of synapse structural plasticity; dopamine receptor, phospholipase C activating pathway
Disease: Myoclonic Dystonia
Research Articles on DRD2
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Product Notes
The DRD2 drd2 (Catalog #AAA618780) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Dopamine Receptor, D2L, D2S reacts with Human, Monkey, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Dopamine Receptor, D2L, D2S can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC). Suitable for use in ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry. Dilution: ELISA: 0.5-1.0ug/ml Western Blot: 1:1000. Detected Dopamine Receptor D2 on 10ug of human brain lysates. Immunoprecipitation: 1-10ug/ml. Use 100-400ug of protein. An antibody made to this epitope has been shown to immunoprecipitate both the long and short forms. Immunohistochemistry (Paraformaldehyde fixed sections): 2-10ug/ml. Researchers should empirically determine the suitability of the DRD2 drd2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Dopamine Receptor, D2L, D2S, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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