Typical Testing Data/Standard Curve (for reference only)
Human Ectonucleoside Triphosphate Diphosphohydrolase 1 (ENTPD1) ELISA Kit | ENTPD1 elisa kit
Human Ectonucleoside Triphosphate Diphosphohydrolase 1 (ENTPD1) ELISA Kit
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to ENTPD1. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to ENTPD1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ENTPD1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of ENTPD1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
has apyrase activity; may inactivate signaling mediated by extracellular nucleotides [RGD, Feb 2006]
Uniprot Description
ENTPD1: In the nervous system, could hydrolyze ATP and other nucleotides to regulate purinergic neurotransmission. Could also be implicated in the prevention of platelet aggregation by hydrolyzing platelet-activating ADP to AMP. Hydrolyzes ATP and ADP equally well. Belongs to the GDA1/CD39 NTPase family. 5 isoforms of the human protein are produced by alternative splicing.
Protein type: Nucleotide Metabolism - purine; Membrane protein, multi-pass; Nucleotide Metabolism - pyrimidine; EC 3.6.1.5; Extracellular matrix; Membrane protein, integral; Hydrolase
Cellular Component: extracellular space; synaptic vesicle; neuron projection; cell surface; cell soma; membrane; basolateral plasma membrane; postsynaptic density; integral to membrane; basal lamina
Molecular Function: identical protein binding; hydrolase activity; nucleoside-diphosphatase activity; ATPase activity; nucleoside-triphosphatase activity; ATP binding; adenosine-diphosphatase activity
Biological Process: G-protein coupled receptor protein signaling pathway; platelet activation; metabolic process; response to proline; response to morphine; response to gamma radiation; response to lipopolysaccharide; negative regulation of dopamine secretion; purine ribonucleoside diphosphate catabolic process; protein homooligomerization; response to ATP; response to caffeine
Research Articles on ENTPD1
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Product Notes
The Human ENTPD1 entpd1 (Catalog #AAA2024743) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2024743 ELISA Kit recognizes Human ENTPD1. It is sometimes possible for the material contained within the vial of "Ectonucleoside Triphosphate Diphosphohydrolase 1 (ENTPD1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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