Angiopoietin 4 (ANGPT4) Recombinant Protein | ANGPT4 recombinant protein

Recombinant Angiopoietin 4 (ANGPT4)

Cat. Num. AAA2009897

• Gene Names: Ang4; Raa4; Rnase5d
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Angiopoietin 4 (ANGPT4); Recombinant Angiopoietin 4 (ANGPT4); ANGPT4 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and GST-tag, its sequence is listed below.; MRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGSEF- FLRQHYDAK PNGRDDRYCE SMMKERKLTS PCKDVNTFIH GTKKNIRAIC GKKGSPYGEN FRISNSPFQI TTCTHSGASP RPPCGYRAFK DFRYIVIACE DGWPVHFDES FISP
• Sequence Length: 144

• Applicable Applications for ANGPT4 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Mus musculus (Mouse)
• Expression System: Prokaryotic expression
• Residues: Phe32~Pro144 (Accession # Q3TMQ6) with two N-terminal Tags, His-tag and GST-tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 157388977
• NCBI GeneID: 219033
• NCBI Accession #: NP_808212.2
• NCBI GenBank Nucleotide #: NM_177544.4
• UniProt Accession #: Q3TMQ6; Q3UWE7; Q80XS4; Q80Z85
• Molecular Weight: 40.0kDa
• NCBI Official Full Name: angiogenin-4
• NCBI Official Synonym Full Names: angiogenin, ribonuclease A family, member 4
• NCBI Official Symbol: Ang4
• NCBI Official Synonym Symbols: Raa4; Rnase5d
• NCBI Protein Information: angiogenin-4; ribonuclease A a4
• UniProt Protein Name: Angiogenin-4
• Protein Family: Angiopoietin
• UniProt Gene Name: Ang4
• UniProt Entry Name: ANG4_MOUSE

Uniprot Description

ANG4: Has bactericidal activity against E.faecalis and L.monocytogenes, but not against L.innocua and E.coli. Promotes angiogenesis (in vitro). Has low ribonuclease activity (in vitro). Promotes proliferation of melanoma cells, but not of endothelial cells or fibroblasts (in vitro). Belongs to the pancreatic ribonuclease family.

Protein type: EC 3.1.27.-

Cellular Component: extracellular space; proteinaceous extracellular matrix; extracellular region; basement membrane; cytoplasmic vesicle; nucleus; secretory granule

Molecular Function: nuclease activity; nucleic acid binding; hydrolase activity; endonuclease activity; ribonuclease activity

Biological Process: defense response to bacterium; RNA catabolic process; positive regulation of cell proliferation; antibacterial humoral response; angiogenesis