Cyclin Dependent Kinase 2 (CDK2) Recombinant Protein | CDK2 recombinant protein

Recombinant Cyclin Dependent Kinase 2 (CDK2)

Cat. Num. AAA2011414

• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Cyclin Dependent Kinase 2 (CDK2); Recombinant Cyclin Dependent Kinase 2 (CDK2); CDK2 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and GST-tag, its sequence is listed below.; MRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGSEF-SALTGLP LPLIKSYLFQ LLQGLAFCHS HRVLHRDLKP QNLLINAEGS IKLADFGLAR AFGVPVRTYT HEVVTLWYRA PEILLGCKYY STAVDIWSLG CIFAEMVTRR ALFPGDSEID QL
• Sequence Length: 298

• Applicable Applications for CDK2 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Rattus norvegicus (Rat)
• Expression System: Prokaryotic expression
• Residues: Ser94~Leu212 (Accession # Q63699) with two N-terminal Tags, His-tag and GST-tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 6166046
• NCBI GeneID: 362817
• UniProt Accession #: Q63699; O09136
• Molecular Weight: 40.4kDa
• NCBI Official Full Name: Cyclin-dependent kinase 2
• NCBI Official Synonym Full Names: cyclin dependent kinase 2
• NCBI Official Symbol: Cdk2
• NCBI Protein Information: cyclin-dependent kinase 2; cell division protein kinase 2; cyclin dependent kinase 2-alpha
• UniProt Protein Name: Cyclin-dependent kinase 2
• Protein Family: Cyclin-dependent kinase
• UniProt Gene Name: Cdk2
• UniProt Synonym Gene Names: Cdkn2
• UniProt Entry Name: CDK2_RAT

Uniprot Description

CDK2: a protein kinase of the CDK family. An important component of the cell cycle machinery. Activity of CDK2 is maximal during S phase and G2. Cdk2/cyclin E kinase activity is important for the G1 to S transition and phosphorylates the Rb protein. In S-phase, active cdk2/cyclin A complexes predominate and phosphorylate E2F, and the active cdk2 complex persists in the nucleus through G2. Part of the Rb pathway disregulated in most tumors. Target of several candidate cancer drugs. However, inhibition does not always prevent cancer cell growth, possibly due to CDK redundancy. Inhibitors: BMS-265246, BMS-265246-01, R-roscovitine (CYC200, CYC202), SU9516, R547, L868276

Protein type: EC 2.7.11.22; Cell cycle regulation; Protein kinase, CMGC; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); CMGC group; CDK family; CDK/CDK1 subfamily; CDK1 subfamily

Cellular Component: Y chromosome; Cajal body; chromosome, telomeric region; transcription factor complex; intracellular membrane-bound organelle; X chromosome; cytoplasm; cyclin-dependent protein kinase holoenzyme complex; microtubule organizing center; condensed chromosome; cytosol; nucleus; endosome

Molecular Function: cyclin binding; cyclin-dependent protein kinase activity; metal ion binding; protein complex binding; histone kinase activity; kinase activity; ATP binding; protein kinase activity

Biological Process: response to drug; lens development in camera-type eye; mitosis; organ regeneration; response to cAMP; regulation of cell cycle; response to toxin; positive regulation of transcription, DNA-dependent; histone phosphorylation; DNA repair; protein amino acid phosphorylation; response to estradiol stimulus; peptidyl-serine phosphorylation; response to cadmium ion; response to ethanol; meiotic cell cycle; cellular response to insulin stimulus; cell division; Ras protein signal transduction; positive regulation of cell proliferation; response to electrical stimulus; potassium ion transport; G1/S transition of mitotic cell cycle; positive regulation of DNA replication initiation