Fibulin 4 (FBLN4) Recombinant Protein | FBLN4 recombinant protein

Recombinant Fibulin 4 (FBLN4)

Cat. Num. AAA2010668

• Gene Names: EFEMP2; MBP1; UPH1; FBLN4; ARCL1B
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Fibulin 4 (FBLN4); Recombinant Fibulin 4 (FBLN4); FBLN4 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and GST-tag, its sequence is listed below.; MRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGSEF- TRL CQDIDECESG AHQCSEAQTC VNFHGGYRCV DTNRCVEPYI QVSENRCLCP ASNPLCREQP SSIVHRYMTI TSERSVPADV FQIQAT
• Sequence Length: 443

• Applicable Applications for FBLN4 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Homo sapiens (Human)
• Expression System: Prokaryotic expression
• Residues: Thr278~Thr366 (Accession # O95967) with two N-terminal Tags, His-tag and GST-tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 320118911
• NCBI GeneID: 30008
• NCBI Accession #: NP_058634.4
• NCBI GenBank Nucleotide #: NM_016938.4
• UniProt Accession #: O95967; O75967; A8K7R4; B3KM31; B3KQT1
• Molecular Weight: 37.0kDa
• NCBI Official Full Name: EGF-containing fibulin-like extracellular matrix protein 2
• NCBI Official Synonym Full Names: EGF containing fibulin-like extracellular matrix protein 2
• NCBI Official Symbol: EFEMP2
• NCBI Official Synonym Symbols: MBP1; UPH1; FBLN4; ARCL1B
• NCBI Protein Information: EGF-containing fibulin-like extracellular matrix protein 2; FIBL-4; fibulin 4; fibulin-4; mutant p53 binding protein 1
• UniProt Protein Name: EGF-containing fibulin-like extracellular matrix protein 2
• UniProt Gene Name: EFEMP2
• UniProt Synonym Gene Names: FBLN4; FIBL-4
• UniProt Entry Name: FBLN4_HUMAN

NCBI Description

A large number of extracellular matrix proteins have been found to contain variations of the epidermal growth factor (EGF) domain and have been implicated in functions as diverse as blood coagulation, activation of complement and determination of cell fate during development. The protein encoded by this gene contains four EGF2 domains and six calcium-binding EGF2 domains. This gene is necessary for elastic fiber formation and connective tissue development. Defects in this gene are cause of an autosomal recessive cutis laxa syndrome. Alternatively spliced transcript variants have been identified for this gene. [provided by RefSeq, Jan 2011]

Uniprot Description

EFEMP2: Defects in EFEMP2 are the cause of cutis laxa, autosomal recessive, type 1B (ARCL1B). A connective tissue disorder characterized by characterized by loose, hyperextensible skin with decreased resilience and elasticity leading to a premature aged appearance. Face, hands, feet, joints, and torso may be differentially affected. The clinical spectrum of autosomal recessive cutis laxa is highly heterogeneous with respect to organ involvement and severity. ARCL1B features include emphysema, lethal pulmonary artery occlusion, aortic aneurysm, cardiopulmonary insufficiency, birth fractures, arachnodactyly, and fragility of blood vessels. Belongs to the fibulin family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 11q13.1

Cellular Component: plasma membrane; extracellular region; basement membrane; vesicle

Molecular Function: protein binding; transmembrane receptor activity; extracellular matrix structural constituent; calcium ion binding

Biological Process: extracellular matrix organization and biogenesis; signal transduction; blood coagulation

Disease: Cutis Laxa, Autosomal Recessive, Type Ib