Cholecystokinin (CCK) Recombinant Protein | CCK recombinant protein

Recombinant Cholecystokinin (CCK)

Cat. Num. AAA2009882

• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Cholecystokinin (CCK); Recombinant Cholecystokinin (CCK); CCK recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and GST-tag, its sequence is listed below.; MRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGSEF-MKSGVCLCVV MAVLAAGALA QPVVPAEATD PVEQRAQEAP RRQLRAVLRT DGEPRARLGA LLARYIQQVR KAPSGRMSVL KNLQSLDPSH RISDRDYMGW MDFGRRSAED YEYPS
• Sequence Length: 134

• Applicable Applications for CCK recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Mus musculus (Mouse)
• Expression System: Prokaryotic expression
• Residues: Met1~Ser115 (Accession # P09240) with two N-terminal Tags, His-tag and GST-tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 548962138
• NCBI GeneID: 12424
• NCBI Accession #: NP_001271437.1
• NCBI GenBank Nucleotide #: NM_001284508.1
• UniProt Accession #: P09240; Q53WS9; Q9DCL5
• Molecular Weight: 39.8kDa
• NCBI Official Full Name: cholecystokinin isoform 2
• NCBI Official Synonym Full Names: cholecystokinin
• NCBI Official Symbol: Cck
• NCBI Protein Information: cholecystokinin
• UniProt Protein Name: Cholecystokinin
• Protein Family: Cholecystokinin
• UniProt Gene Name: Cck
• UniProt Synonym Gene Names: CCK; CCK33; CCK12; CCK8
• UniProt Entry Name: CCKN_MOUSE

NCBI Description

This gene encodes a member of the gastrin/cholecystokinin family and preproprotein that is proteolytically processed to generate multiple protein products. These products include the peptide hormones cholecystokinin-8, -12, -33, and others. The encoded peptides have been shown to regulate gastric acid secretion and food intake. Homozygous knockout mice for this gene exhibit impaired insulin secretion, enhanced insulin sensitivity, and resistance to obesity. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2015]

Uniprot Description

CCK: This peptide hormone induces gall bladder contraction and the release of pancreatic enzymes in the gut. Its function in the brain is not clear. Binding to CCK-A receptors stimulates amylase release from the pancreas, binding to CCK-B receptors stimulates gastric acid secretion. Belongs to the gastrin/cholecystokinin family.

Protein type: Secreted; Hormone; Cell development/differentiation; Secreted, signal peptide

Cellular Component: axon hillock; extracellular space; cell soma; axon; dendrite; extracellular region; terminal button; perikaryon

Molecular Function: neuropeptide hormone activity; protein binding; hormone activity

Biological Process: caspase activation; release of cytochrome c from mitochondria; behavioral fear response; eating behavior; positive regulation of apoptosis; apoptosis; neuron migration; negative regulation of appetite; positive regulation of protein oligomerization; positive regulation of peptidyl-tyrosine phosphorylation; axonogenesis; positive regulation of mitochondrial depolarization; positive regulation of cell proliferation; protein kinase C activation; regulation of sensory perception of pain