Typical Testing Data/Standard Curve (for reference only)
Mouse alpha-Amylase ELISA Kit | AMS/AMY elisa kit
Mouse alpha-Amylase ELISA Kit
Gene Names
malS; ECK3560; JW3543
Reactivity
Mouse
Synonyms
alpha-Amylase; Mouse alpha-Amylase ELISA Kit; a-Amylase; α-Amylase; alpha-Amylase (Mouse); AMS/AMY elisa kit
Reactivity
Mouse
Samples
Cell culture fluid, body fluid, tissue homogenate, serum and blood plasma
Preparation and Storage
Store all reagents at 2-8 degree C
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for AMS/AMY elisa kit
For Samples: Cell culture fluid, body fluid, tissue homogenate, serum and blood plasma
Intended Uses: This AAM ELISA kit is intended for laboratory research use only. The stop solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of AAM in the sample, this AAM ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus AAM concentration. The concentration of in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The coated well immunoenzymatic assay for the quantitative measurement of AAM utilizes a multiclonal anti-AAM antibody and an AAM-HRP conjugate. The assay sample and buffer are incubated together with AAM-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the AAM concentration since AAM from samples and AAM-HRP conjugate compete for the anti-AAM antibody binding site. Since the number of sites is limited, as more sites are occupied by AAM from the sample, fewer sites are left to bind AAM-HRP conjugate. Standards of known AAM concentrations are run concurrently with the samples being assayed and a standard curve is plotted relating the intensity of the color (Optical Density) to the concentration of AAM. The AAM concentration in each sample is interpolated from this standard curve.
Intended Uses: This AAM ELISA kit is intended for laboratory research use only. The stop solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of AAM in the sample, this AAM ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus AAM concentration. The concentration of in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The coated well immunoenzymatic assay for the quantitative measurement of AAM utilizes a multiclonal anti-AAM antibody and an AAM-HRP conjugate. The assay sample and buffer are incubated together with AAM-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the AAM concentration since AAM from samples and AAM-HRP conjugate compete for the anti-AAM antibody binding site. Since the number of sites is limited, as more sites are occupied by AAM from the sample, fewer sites are left to bind AAM-HRP conjugate. Standards of known AAM concentrations are run concurrently with the samples being assayed and a standard curve is plotted relating the intensity of the color (Optical Density) to the concentration of AAM. The AAM concentration in each sample is interpolated from this standard curve.
Product Categories/Family for AMS/AMY elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
75,713 Da
NCBI Official Full Name
alpha-amylase
NCBI Official Symbol
malS
NCBI Official Synonym Symbols
ECK3560; JW3543
NCBI Protein Information
alpha-amylase
UniProt Protein Name
Alpha-amylase
Protein Family
UniProt Gene Name
malS
UniProt Entry Name
AMY1_ECOLI
NCBI Description
MalT regulon. [More information is available at EcoGene: EG11316]. MalS is a periplasmic enzyme that degrades linear dextrins of at least three glucose residues. [More information is available at EcoCyc: EG11316].
Uniprot Description
Function: Since only maltooligosaccharides up to a chain length of 6 glucose units are actively transported through the cytoplasmic membrane via the membrane-bound complex of three proteins, MalF, MalG, and MalK, longer maltooligosaccharides must first be degraded by the periplasmic alpha-amylase, the MalS protein.
Catalytic activity: Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D-glucose units.
Cofactor: Binds 1 calcium ion per subunit
By similarity.
Subunit structure: Monomer.
Subcellular location: Periplasm.
Induction: Under the regulatory control of the MalT protein.
Sequence similarities: Belongs to the glycosyl hydrolase 13 family.
Research Articles on AMS/AMY
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Product Notes
The Mouse AMS/AMY mals (Catalog #AAA730160) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA730160 ELISA Kit recognizes Mouse AMS/AMY. It is sometimes possible for the material contained within the vial of "alpha-Amylase, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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