Human alpha2 antiplasmin ELISA Kit | alpha2-AP elisa kit

Human alpha2 antiplasmin ELISA Kit

Cat. Num. AAA730931

• Gene Names: SERPINF2; serpin
• Reactivity: Human
• Synonyms: alpha2 antiplasmin; Human alpha2 antiplasmin ELISA Kit; a2 antiplasmin; α2 antiplasmin; alpha2 antiplasmin (Human); alpha2-AP elisa kit

• Reactivity: Human

• Samples: Cell culture fluid & body fluid & tissue homogenate Serum or blood plasma
• Preparation and Storage: Store all reagents at 2-8 degree C

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for alpha2-AP elisa kit

For Samples: Cell culture fluid & body fluid & tissue homogenate Serum or blood plasma

Intended Uses: This A2-AP ELISA kit is intended for laboratory research use only. The stop solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of A2-AP in the sample, this A2-AP ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus A2-AP concentration. The concentration of in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Principle of the Assay: The coated well immunoenzymatic assay for the quantitative measurement of A2-AP utilizes a multiclonal anti-A2-AP antibody and an A2-AP-HRP conjugate. The assay sample and buffer are incubated together with A2-AP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the A2-AP concentration since A2-AP from samples and A2-AP-HRP conjugate compete for the anti-A2-AP antibody binding site. Since the number of sites is limited, as more sites are occupied by A2-AP from the sample, fewer sites are left to bind A2-AP-HRP conjugate. Standards of known A2-AP concentrations are run concurrently with the samples being assayed and a standard curve is plotted relating the intensity of the color (Optical Density) to the concentration of A2-AP. The A2-AP concentration in each sample is interpolated from this standard curve.

Product Categories/Family for alpha2-AP elisa kit

Human ELISA Kit

NCBI and Uniprot Product Information

• NCBI GI #: 130488651
• NCBI GeneID: 100009515
• NCBI Accession #: NP_001076210.1
• NCBI GenBank Nucleotide #: NM_001082741.2
• NCBI Official Full Name: alpha-2-antiplasmin
• NCBI Official Symbol: SERPINF2
• NCBI Official Synonym Symbols: serpin
• NCBI Protein Information: alpha-2-antiplasmin; alpha-2-plasmin inhibitor; alpha-2-PI serine protease inhibitor