Typical Testing Data/Standard Curve (for reference only)
Mouse cytochrome b561 ELISA Kit | Cytb561 elisa kit
Mouse cytochrome b561 ELISA Kit
Reactivity
Mouse
Synonyms
cytochrome b561; Mouse cytochrome b561 ELISA Kit; Mouse cytochrome b561 (Cytb561) ELISA Kit; cytochrome b561 (Mouse); Cytb561 elisa kit
Reactivity
Mouse
Samples
Cell culture fluid, body fluid, tissue homogenate, serum and blood plasma
Preparation and Storage
Store all reagents at 2-8 degree C
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for Cytb561 elisa kit
For Samples: Cell culture fluid, body fluid, tissue homogenate, serum and blood plasma
Intended Uses: This CYB561 ELISA kit is intended for laboratory research use only. The stop solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of CYB561 in the sample, this CYB561 ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus CYB561 concentration. The concentration of in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The coated well immunoenzymatic assay for the quantitative measurement of CYB561 utilizes a multiclonal anti-CYB561 antibody and an CYB561-HRP conjugate. The assay sample and buffer are incubated together with CYB561-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CYB561 concentration since CYB561 from samples and CYB561-HRP conjugate compete for the anti-CYB561 antibody binding site. Since the number of sites is limited, as more sites are occupied by CYB561 from the sample, fewer sites are left to bind CYB561-HRP conjugate. Standards of known CYB561 concentrations are run concurrently with the samples being assayed and a standard curve is plotted relating the intensity of the color (Optical Density) to the concentration of CYB561. The CYB561 concentration in each sample is interpolated from this standard curve.
Intended Uses: This CYB561 ELISA kit is intended for laboratory research use only. The stop solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of CYB561 in the sample, this CYB561 ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus CYB561 concentration. The concentration of in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The coated well immunoenzymatic assay for the quantitative measurement of CYB561 utilizes a multiclonal anti-CYB561 antibody and an CYB561-HRP conjugate. The assay sample and buffer are incubated together with CYB561-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CYB561 concentration since CYB561 from samples and CYB561-HRP conjugate compete for the anti-CYB561 antibody binding site. Since the number of sites is limited, as more sites are occupied by CYB561 from the sample, fewer sites are left to bind CYB561-HRP conjugate. Standards of known CYB561 concentrations are run concurrently with the samples being assayed and a standard curve is plotted relating the intensity of the color (Optical Density) to the concentration of CYB561. The CYB561 concentration in each sample is interpolated from this standard curve.
Product Categories/Family for Cytb561 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
cytochrome b561
NCBI Official Symbol
cytb561
NCBI Protein Information
cytochrome b561
Protein Family
Similar Products
Product Notes
The Mouse Cytb561 (Catalog #AAA726150) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA726150 ELISA Kit recognizes Mouse Cytb561. It is sometimes possible for the material contained within the vial of "cytochrome b561, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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