Typical Testing Data/Standard Curve (for reference only)
Rat Amylase Alpha 2, Pancreatic ELISA Kit | AMY2 elisa kit
Rat Amylase Alpha 2, Pancreatic ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: AMYalpha2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-AMYalpha2 antibody and an AMYalpha2-HRP conjugate. The assay sample and buffer are incubated together with AMYalpha2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the AMYalpha2 concentration since AMYalpha2 from samples and AMYalpha2-HRP conjugate compete for the anti-AMYalpha2 antibody binding site. Since the number of sites is limited, as more sites are occupied by AMYalpha2 from the sample, fewer sites are left to bind AMYalpha2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AMYalpha2 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
Amylases are secreted proteins that hydrolyze 1,4-alpha-glucoside bonds in oligosaccharides and polysaccharides, and thus catalyze the first step in digestion of dietary starch and glycogen. The human genome has a cluster of several amylase genes that are expressed at high levels in either salivary gland or pancreas. This gene encodes an amylase isoenzyme produced by the pancreas. [provided by RefSeq, Jul 2008]
Uniprot Description
AMY2A: Amylases are secreted proteins that hydrolyze 1,4-alpha-glucoside bonds in oligosaccharides and polysaccharides, and thus catalyze the first step in digestion of dietary starch and glycogen. The human genome has a cluster of several amylase genes that are expressed at high levels in either salivary gland or pancreas. This gene encodes an amylase isoenzyme produced by the pancreas. [provided by RefSeq, Jul 2008]
Protein type: EC 3.2.1.1; Secreted; Secreted, signal peptide; Hydrolase; Carbohydrate Metabolism - starch and sucrose
Chromosomal Location of Human Ortholog: 1p21
Cellular Component: extracellular space; extracellular region
Molecular Function: alpha-amylase activity; calcium ion binding; chloride ion binding
Biological Process: polysaccharide digestion; carbohydrate catabolic process; carbohydrate metabolic process; pathogenesis
Research Articles on AMY2
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Product Notes
The Rat AMY2 amy2a (Catalog #AAA750781) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA750781 ELISA Kit recognizes Rat AMY2. It is sometimes possible for the material contained within the vial of "Amylase Alpha 2, Pancreatic , ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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