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alpha amylase/AMY2A Recombinant Protein | AMY2A recombinant protein
alpha amylase/AMY2A Protein, Rhesus, Recombinant (hFc Tag)
Purity
>90% as determined by SDS-PAGE
Synonyms
alpha amylase/AMY2A; alpha amylase/AMY2A Protein; Rhesus; Recombinant (hFc Tag); Rhesus AMY2A/Alpha-amylase Protein (Fc Tag); AMY2A Protein; AMY2B Protein; amylase; alpha 2A (pancreatic); AMY2A recombinant protein
Host
HEK293 Cells
Purity/Purification
>90% as determined by SDS-PAGE
Form/Format
Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
Sequence
Met1-Leu511
Species
Rhesus
Endotoxin
<1.0EU per ug of the protein as determined by the LAL method
Predicted N Terminal
Gln 16
Tag
C-human IgG1-Fc
Protein Construction
A DNA sequence encoding the rhesus AMY2A (H9EX43)(Met1-Leu511) was expressed with the Fc region of human IgG1 at the C-terminus.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Preparation and Storage
Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.
They are shipped out with blue ice.
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.
They are shipped out with blue ice.
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SDS-Page
Related Product Information for AMY2A recombinant protein
Background: Alpha-amylase is the major form of amylase found in humans and other mammals. Amylases are secreted proteins that hydrolyze 1,4-alpha-glucoside bonds in oligosaccharides and polysaccharides, and thus catalyze the first step in digestion of dietary starch and glycogen. Alpha-amylase hydrolyses alpha bonds of large, alpha-linked polysaccharides, such as starch and glycogen, yielding glucose and maltose. Amylases is widely expressed and is most prominent in pancreatic juice and saliva, each of which has its own isoform of human alpha-amylase. They behave differently on isoelectric focusing, and can also be separated in testing by using specific monoclonal antibodies.
References
Abe A, et al. (2005) Complexes of Thermoactinomyces vulgaris R-47 Alpha-amylase/AMY2A 1 and pullulan model oligossacharides provide new insight into the mechanism for recognizing substrates with alpha-(1,6) glycosidic linkages. FEBS J. 272(23):6145-53.Aghajari, N, et al. (1998) Crystal structures of the psychrophilic Alpha-amylase/AMY2A from Alteromonas haloplanctis in its native form and complexed with an inhibitor. Protein Sci. 7(3): 564-72.Ramasubbu, N, et al. (1996) Structure of Human Salivary -Amylase at 1.6 Resolution: Implications for its Role in the Oral Cavity. Acta Crystallographica Section D Biological Crystallography. 52(3):435-46.
NCBI and Uniprot Product Information
UniProt Accession #
Molecular Weight
The recombinant rhesus AMY2A comprises 737 amino acids and has a calculated molecular mass of 82.8kDa.
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Product Notes
The AMY2A (Catalog #AAA8123523) is a Recombinant Protein produced from HEK293 Cells and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: Met1-Leu51 1. It is sometimes possible for the material contained within the vial of "alpha amylase/AMY2A, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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