Rabbit anti-Human SIRPB2 Polyclonal Antibody | anti-SIRPB2 antibody

SIRPB2, CT (SIRPB2, PTPN1L, PTPNS1L3, Signal-regulatory protein beta-2, Protein tyrosine phosphatase non-receptor type substrate 1-like 3, Protein tyrosine phosphatase non-receptor type substrate protein) (FITC)

Cat. Num. AAA6347761

• Gene Names: SIRPB2; PTPN1L; PTPNS1L3; dJ776F14.2
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: SIRPB2; Polyclonal Antibody; CT (SIRPB2; PTPN1L; PTPNS1L3; Signal-regulatory protein beta-2; Protein tyrosine phosphatase non-receptor type substrate 1-like 3; Protein tyrosine phosphatase non-receptor type substrate protein) (FITC); Protein tyrosine phosphatase non-receptor type substrate protein; anti-SIRPB2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-SIRPB2 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: SIRPB2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 228-255 amino acids from the C-terminal region of human SIRPB2.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(SIRPB2 Antibody (C-term) western blot analysis in ZR-75-1 cell line lysates (35ug/lane).This demonstrates the SIRPB2 antibody detected the SIRPB2 protein (arrow).)

Related Product Information for anti-SIRPB2 antibody

Probable immunoglobulin-like cell surface receptor. On binding with CD47, mediates cell-cell adhesion. Engagement on T-cells by CD47 on antigen-presenting cells results in enhanced antigen-specific T-cell proliferation and costimulates T-cell activation.

Product Categories/Family for anti-SIRPB2 antibody

Antibodies; Enzymes; Phosphatase

References

Nyati, M.K., et al. Cancer Res. 66(24):11554-11559(2006). van den Berg, T.K., et al. J. Immunol. 175(12):7788-7789(2005). Chen, J., et al. Proc. Natl. Acad. Sci. U.S.A. 99(19):12257-12262(2002).

NCBI and Uniprot Product Information

• NCBI GI #: 171906611
• NCBI GeneID: 284759
• NCBI Accession #: NP_001116434.1
• NCBI GenBank Nucleotide #: NM_001122962.1
• UniProt Accession #: Q5JXA9; Q6TCH9; B4DLM9; E9PCW6
• Molecular Weight: 36,968 Da
• NCBI Official Full Name: signal-regulatory protein beta-2 isoform 1
• NCBI Official Synonym Full Names: signal-regulatory protein beta 2
• NCBI Official Symbol: SIRPB2
• NCBI Official Synonym Symbols: PTPN1L; PTPNS1L3; dJ776F14.2
• NCBI Protein Information: signal-regulatory protein beta-2; SIRP-beta-2; protein tyrosine phosphatase non-receptor type substrate protein; protein tyrosine phosphatase non-receptor type substrate 1-like 3; protein tyrosine phosphatase, non-receptor type substrate 1-like 3
• UniProt Protein Name: Signal-regulatory protein beta-2
• Protein Family: Signal-regulatory protein
• UniProt Gene Name: SIRPB2
• UniProt Synonym Gene Names: PTPN1L; PTPNS1L3; SIRP-beta-2
• UniProt Entry Name: SIRB2_HUMAN

Uniprot Description

SIRPB2: 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 20p13

Cellular Component: integral to membrane