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beta Amylase/AMY2B Recombinant Protein | AMY2B recombinant protein
beta Amylase/AMY2B Protein, Human, Recombinant (His Tag)
Gene Names
AMY2B; HXA; AMY2; AMY3
Purity
>95% as determined by SDS-PAGE
Synonyms
beta Amylase/AMY2B; beta Amylase/AMY2B Protein; Human; Recombinant (His Tag); Human AMY2B Protein (His Tag); AMY2 Protein; AMY3 Protein; HXA Protein; amylase; alpha 2B (pancreatic); AMY2B recombinant protein
Host
HEK293 Cells
Purity/Purification
>95% as determined by SDS-PAGE
Form/Format
Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
Sequence
Met1-Leu511
Species
Human
Endotoxin
<1.0EU per ug protein as determined by the LAL method.
Predicted N Terminal
Gln 16
Tag
C-His
Protein Construction
A DNA sequence encoding the human AMY2B (NP_066188.1)(Met1-Leu511) was expressed with a polyhistidine tag at the C-terminus.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Preparation and Storage
Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.
They are shipped out with blue ice.
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.
They are shipped out with blue ice.
SDS-Page
SDS-Page
Related Product Information for AMY2B recombinant protein
Background: Amylases are secreted proteins that hydrolyze 1,4-alpha-glucoside bonds in oligosaccharides and polysaccharides, and thus catalyze the first step in digestion of dietary starch and glycogen. Alpha-amylase is the major form of amylase found in humans and other mammals. Alpha-amylase hydrolyses alpha bonds of large, alpha-linked polysaccharides, such as starch and glycogen, yielding glucose and maltose. Amylases is widely expressed and is most prominent in pancreatic juice and saliva, each of which has its own isoform of human alpha-amylase. They behave differently on isoelectric focusing, and can also be separated in testing by using specific monoclonal antibodies.
References
Abe A. et al., 2005, FEBS J. 272 (23): 6145-53. Aghajari N. et al., 1998, Protein Sci. 7 (3): 564-72. Ramasubbu N. et al., 1996, Acta Crystallographica Section D Biological Crystallography. 52 (3): 435-46.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
42,021 Da
NCBI Official Full Name
alpha-amylase 2B
NCBI Official Synonym Full Names
amylase, alpha 2B (pancreatic)
NCBI Official Symbol
AMY2B
NCBI Official Synonym Symbols
HXA; AMY2; AMY3
NCBI Protein Information
alpha-amylase 2B
UniProt Protein Name
Alpha-amylase 2B
Protein Family
UniProt Gene Name
AMY2B
UniProt Entry Name
AMY2B_HUMAN
NCBI Description
Amylases are secreted proteins that hydrolyze 1,4-alpha-glucoside bonds in oligosaccharides and polysaccharides, and thus catalyze the first step in digestion of dietary starch and glycogen. The human genome has a cluster of several amylase genes that are expressed at high levels in either salivary gland or pancreas. This gene encodes an amylase isoenzyme produced by the pancreas. [provided by RefSeq, Jun 2013]
Uniprot Description
AMY2B: Monomer. Belongs to the glycosyl hydrolase 13 family.
Protein type: Carbohydrate Metabolism - starch and sucrose; Secreted, signal peptide; EC 3.2.1.1; Secreted; Hydrolase
Chromosomal Location of Human Ortholog: 1p21
Molecular Function: alpha-amylase activity; metal ion binding
Biological Process: carbohydrate metabolic process; digestion
Research Articles on AMY2B
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Product Notes
The AMY2B amy2b (Catalog #AAA8121470) is a Recombinant Protein produced from HEK293 Cells and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: Met1-Leu51 1. It is sometimes possible for the material contained within the vial of "beta Amylase/AMY2B, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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