Rabbit Dopamine Receptor D5 Polyclonal Antibody | anti-DRD5 antibody

Anti-Dopamine Receptor D5 antibody

Cat. Num. AAA176794

• Gene Names: Drd5; D5R; Drd-5; Drd1b; Gpcr1
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: Immunogen affinity purified.
• Synonyms: Dopamine Receptor D5; Polyclonal Antibody; Anti-Dopamine Receptor D5 antibody; D(1B) dopamine receptor; dopamine receptor D5; D(1B) dopamine receptor antibody; D(5) dopamine receptor antibody; D1 beta dopamine receptor antibody; D1B dopamine receptor antibody; D1beta dopamine receptor antibody; D5 dopamine receptor antibody; DBDR antibody; Dopamine D5 receptor antibody; Dopamine receptor D1B antibody; DRD 1B antibody; DRD 5 antibody; DRD1B antibody; DRD1L2 antibody; DRD5 antibody; DRD5_HUMAN antibody; MGC10601 antibody; anti-DRD5 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: No cross reactivity with other proteins.
• Purity/Purification: Immunogen affinity purified.
• Form/Format: Lyophilized; Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na₂HPO₄, 0.05mg Thimerosal, 0.05mg NaN₃.
• Sequence Length: 478

• Applicable Applications for anti-DRD5 antibody: Western Blot (WB)
• Application Notes: Western Blot: Concentration: 0.1-0.5 ug/ml; Tested Species: Human, Mouse, Rat; ; Tested Species: In house tested species with positive test results.; ; Predicted Species: Species predicted to be fit for the product based on sequence similarities.; ; Other applicaitons have not been tested.; Optimal dilutions should be determined by end users.
• Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of mouse Dopamine Receptor D5 (388-407aa LISYNQDTVFHREIAAAYVH), different from the related rat sequence by two amino acids, and from the related human sequence by three amino acids.
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Preparation and Storage: At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

Western Blot (WB)

(Anti-Dopamine Receptor D5 antibody, MBS176794, Western blottingAll lanes: Anti Dopamine Receptor D5 (MBS176794) at 0.5ug/mlLane 1: Rat Brain Tissue Lysate at 50ugLane 2: Mouse Brain Rat Brain Tissue Lysate at 50ugLane 3: U87 Whole Cell Lysate at 40ugPredicted bind size: 53KDObserved bind size: 58KD)

Related Product Information for anti-DRD5 antibody

Background: D(1B) dopamine receptor(DRD5), also called DRD1B or DRD1L2 is a protein that in humans is encoded by the DRD5 gene. This gene is mapped to 4p16.1. This gene encodes the D5 subtype of the dopamine receptor. The D5 subtype is a G-protein coupled receptor which stimulates adenylyl cyclase. This receptor is expressed in neurons in the limbic regions of the brain. It has a 10-fold higher affinity for dopamine than the D1 subtype. Pseudogenes related to this gene reside on chromosomes 1 and 2. Compared with DRD1, DRD5 displayed a higher affinity for dopamine and was able to stimulate a biphasic rather than a monophasic intracellular accumulation of cAMP.

NCBI and Uniprot Product Information

• NCBI GI #: 47115791
• NCBI GeneID: 13492
• UniProt Accession #: Q8BLD9; Q61439; Q80UB7
• NCBI Official Full Name: D(1B) dopamine receptor
• NCBI Official Synonym Full Names: dopamine receptor D5
• NCBI Official Symbol: Drd5
• NCBI Official Synonym Symbols: D5R; Drd-5; Drd1b; Gpcr1
• NCBI Protein Information: D(1B) dopamine receptor; D(1B) dopamine receptor; dopamine receptor 5; dopamine D5 receptor; d(5) dopamine receptor
• UniProt Protein Name: D(1B) dopamine receptor
• Protein Family: D(1B) dopamine receptor
• UniProt Gene Name: Drd5
• UniProt Entry Name: DRD5_MOUSE

Uniprot Description

DRD5: Dopamine receptor whose activity is mediated by G proteins which activate adenylyl cyclase. Defects in DRD5 are a cause of benign essential blepharospasm (BEB). BEB is a primary focal dystonia affecting the orbicularis oculi muscles. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. BEB usually begins in middle age. Initial symptoms include eye irritation and frequent blinking, progressing to involuntary spasms of eyelid closure. Patients have normal eyes. The visual disturbance is due solely to the forced closure of the eyelids. In severe cases, this can lead to functional blindness. Belongs to the G-protein coupled receptor 1 family.

Protein type: Membrane protein, integral; GPCR, family 1; Receptor, GPCR; Membrane protein, multi-pass

Cellular Component: cell soma; membrane; integral to plasma membrane; axon; brush border membrane; dendrite; plasma membrane; dendritic spine; integral to membrane

Molecular Function: G-protein coupled receptor activity; signal transducer activity; dopamine receptor activity; dopamine binding; G-protein alpha-subunit binding; drug binding; dopamine D1 receptor-like receptor activity

Biological Process: regulation of long-term neuronal synaptic plasticity; synaptic transmission, dopaminergic; wound healing; response to amphetamine; mating behavior; transmission of nerve impulse; norepinephrine-epinephrine vasoconstriction involved in regulation of systemic arterial blood pressure; signal transduction; response to cocaine; G-protein signaling, adenylate cyclase activating pathway; synaptic transmission; G-protein coupled receptor protein signaling pathway; regulation of female receptivity; regulation of systemic arterial blood pressure by vasopressin; positive regulation of cAMP biosynthetic process; sensitization; dopamine receptor signaling pathway; negative regulation of protein kinase activity; visual learning; negative regulation of blood pressure; positive regulation of adenylate cyclase activity; negative regulation of NAD(P)H oxidase activity; negative regulation of cell migration; associative learning; dopamine receptor, adenylate cyclase activating pathway