SDS-Page
(Recombinant AKT3 protein gel 10% SDS-PAGE Coomassie staining MW: 60.8 kDa Purity: ?90%)
AKT3 recombinant protein
Recombinant AKT3 protein
Shipping Temp: Dry Ice
SDS-Page
(Recombinant AKT3 protein gel 10% SDS-PAGE Coomassie staining MW: 60.8 kDa Purity: ?90%)
SDS-Page
(Recombinant AKT3 protein gel 10% SDS-PAGE Coomassie staining MW: 60.8 kDa Purity: ?90%)
Testing Data
(HTRF assay for recombinant AKT3 protein activity 1 uM STK S3 substrate was incubated with different concentrations AKT3 protein in a 10 ul reaction system for 1 hour. The 10 ul detection reagents were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature, and HTRF KinEASE STK assay was used to detect the enzymatic activity.)
Testing Data
(HTRF assay for recombinant AKT3 protein activity 1 uM STK S3 substrate was incubated with different concentrations AKT3 protein in a 10 ul reaction system for 1 hour. The 10 ul detection reagents were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature, and HTRF KinEASE STK assay was used to detect the enzymatic activity.)
Testing Data
(HTRF assay for recombinant AKT3 protein activity 1 uM STK S3 substrate was incubated with different concentrations AKT3 protein in a 10 ul reaction system for 1 hour. The 10 ul detection reagents were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature, and HTRF KinEASE STK assay was used to detect the enzymatic activity.)
Testing Data
(HTRF assay for recombinant AKT3 protein activity 1 uM STK S3 substrate was incubated with different concentrations AKT3 protein in a 10 ul reaction system for 1 hour. The 10 ul detection reagents were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature, and HTRF KinEASE STK assay was used to detect the enzymatic activity.)
Background: AKT3 (AKT Serine/Threonine Kinase 3), also known as Protein Kinase B Gamma or PRKBG, is one of 3 closely related serine/threonine-protein kinases (AKT3, AKT3 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. This is mediated through serine and/or threonine phosphorylation of a range of downstream substrates. Over 100 substrate candidates have been reported so far, but for most of them, no isoform specificity has been reported. AKT3 is the least studied AKT isoform. It plays an important role in brain development and is crucial for the viability of malignant glioma cells. AKT3 isoform may also be the key molecule in up-regulation and down-regulation of MMP13 via IL13. It is required for the coordination of mitochondrial biogenesis with growth factor-induced increases in cellular energy demands. Down-regulation by RNA interference reduces the expression of the phosphorylated form of BAD, resulting in the induction of caspase-dependent apoptosis.
NCBI and Uniprot Product Information
Uniprot Description
Akt3: an AGC kinase that plays a critical role in controlling the balance between survival and apoptosis. Stimulated by platelet-derived growth factor, insulin, and insulin-like growth factor 1. Alternatively spliced isoforms have been described.
Protein type: Protein kinase, AGC; EC 2.7.11.1; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); AGC group; AKT family
Chromosomal Location of Human Ortholog: 1q44
Cellular Component: nucleoplasm; Golgi apparatus; cytoplasm; plasma membrane
Molecular Function: protein serine/threonine kinase activity; protein binding; ATP binding; protein kinase activity
Biological Process: mitochondrial genome maintenance; signal transduction; protein amino acid phosphorylation
Disease: Megalencephaly-polymicrogyria-polydactyly-hydrocephalus Syndrome 2
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Product Notes
The AKT3 akt3 (Catalog #AAA389204) is a Recombinant Protein produced from Baculovirus and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "AKT3, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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