AKT3 recombinant protein

AKT3 Protein, Human, Recombinant (GST Tag)

Cat. Num. AAA8120469

• Gene Names: AKT3; PKBG; PRKBG; STK-2; PKB-GAMMA; RAC-gamma; RAC-PK-gamma
• Purity: >90% as determined by SDS-PAGE
• Synonyms: AKT3; AKT3 Protein; Human; Recombinant (GST Tag); Human AKT3 Protein (GST Tag); MPPH Protein; MPPH2 Protein; PKB-GAMMA Protein; PKBG Protein; PRKBG Protein; RAC-gamma Protein; RAC-PK-gamma Protein; STK-2 Protein; v-akt murine thymoma viral oncogene homolog 3; AKT3 recombinant protein

• Host: Baculovirus-Insect Cells
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Glu479

• Species: Human
• Activity: No Kinase Activity
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Met
• Tag: N-GST
• Protein Construction: A DNA sequence encoding the full length of human PKBG (NP_005456.1)(Met 1-Glu 479) was expressed with the GST tag at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for AKT3 recombinant protein

Background: v-akt murine thymoma viral oncogene homolog 3 (AKT3), also known as PKB-GAMMA, with AKT1/PKBalpha, AKT2/PKBbeta, are the memerbers of Akt kinase family, share extensive structural similarity and perform common as well as unique functions within cells. The Akt signaling cascade initiates at the cell surface when growth factors or other extracellular stimuli activate phosphoinositide 3-kinase (PI3K). AKT3 was discovered to be the predominant isoform activated in sporadic melanomas. Levels of activity increased during melanoma progression with metastatic melanomas having the highest activity. Although mechanisms of AKT3 activation remain to be fully characterized, overexpression of AKT3 and decreased PTEN activity play important roles in this process. Targeted reduction of AKT3 activity decreased survival of melanoma tumor cells leading to inhibition of tumor development, which may be therapeutically effective for shrinking tumors in melanoma patients. AKT2 and AKT3 play an important role in the viability of human malignant glioma cells. Targeting AKT2 and AKT3 may hold promise for the treatment of patients with gliomas.

Product Categories/Family for AKT3 recombinant protein

EGFR Signaling Pathway

References

Mure H, et al. (2010) Akt2 and Akt3 play a pivotal role in malignant gliomas. Neuro Oncol. 12(3): 221-32.Koseoglu S, et al. (2007) AKT1, AKT2 and AKT3-dependent cell survival is cell line-specific and knockdown of all three isoforms selectively induces apoptosis in 20 human tumor cell lines. Cancer Biol Ther. 6(5): 755-62.Cristiano BE, et al. (2006) A specific role for AKT3 in the genesis of ovarian cancer through modulation of G(2)-M phase transition. Cancer Res. 66(24): 11718-25.Robertson GP. (2005) Functional and therapeutic significance of Akt deregulation in malignant melanoma. Cancer Metastasis Rev. 24(2): 273-85.Altomare DA, et al. (2005) Perturbations of the AKT signaling pathway in human cancer. Oncogene. 24: 7455-64.Stahl JM, et al. (2004) Deregulated Akt3 activity promotes development of malignant melanoma. Cancer Res. 64: 7002-10.

NCBI and Uniprot Product Information

• NCBI GI #: 4885549
• NCBI GeneID: 10000
• NCBI Accession #: NP_005456.1; NP_859029.1
• NCBI GenBank Nucleotide #: NM_005465.3
• UniProt Accession #: Q9Y243; Q0VAA6; Q5VTI1; Q5VTI2; Q96QV3; Q9UFP5
• Molecular Weight: 55,775 Da
• NCBI Official Full Name: RAC-gamma serine/threonine-protein kinase isoform 1
• NCBI Official Synonym Full Names: v-akt murine thymoma viral oncogene homolog 3 (protein kinase B, gamma)
• NCBI Official Symbol: AKT3
• NCBI Official Synonym Symbols: PKBG; PRKBG; STK-2; PKB-GAMMA; RAC-gamma; RAC-PK-gamma
• NCBI Protein Information: RAC-gamma serine/threonine-protein kinase; PKB gamma; RAC-gamma serine/threonine protein kinase
• UniProt Protein Name: RAC-gamma serine/threonine-protein kinase
• Protein Family: RAC-gamma serine/threonine-protein kinase
• UniProt Gene Name: AKT3
• UniProt Synonym Gene Names: PKBG; PKB gamma
• UniProt Entry Name: AKT3_HUMAN

Uniprot Description

Akt3: an AGC kinase that plays a critical role in controlling the balance between survival and apoptosis. Stimulated by platelet-derived growth factor, insulin, and insulin-like growth factor 1. Alternatively spliced isoforms have been described.

Protein type: Protein kinase, AGC; EC 2.7.11.1; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); AGC group; AKT family

Chromosomal Location of Human Ortholog: 1q44

Cellular Component: nucleoplasm; Golgi apparatus; cytoplasm; plasma membrane

Molecular Function: protein serine/threonine kinase activity; protein binding; ATP binding; protein kinase activity

Biological Process: mitochondrial genome maintenance; signal transduction; protein amino acid phosphorylation

Disease: Megalencephaly-polymicrogyria-polydactyly-hydrocephalus Syndrome 2