SDS-Page
(SDS-PAGE and Coomassie Stain: Purity was assessed by|SDS-PAGE and Coomassie blue staining using 3ug of A1125-08.)
Akt1, unactivated Recombinant Protein
Akt1, unactivated (v-akt Mouse Thymoma Viral Oncogene Homolog 1, C-AKT, MGC99656, PKB, PRKBA, Protein kinase B, RAC, RAC-ALPHA, RAC-alpha serine/threonine-protein kinase, RAC-PK-alpha) Recombinant
Purity
Highly Purified80%; Purified by SDS-PAGE and Coomassie blue staining.
Synonyms
Akt1; unactivated; unactivated (v-akt Mouse Thymoma Viral Oncogene Homolog 1; C-AKT; MGC99656; PKB; PRKBA; Protein kinase B; RAC; RAC-ALPHA; RAC-alpha serine/threonine-protein kinase; RAC-PK-alpha) Recombinant; unactivated recombinant protein
Host
Recombinant human
Purity/Purification
Highly Purified
80%; Purified by SDS-PAGE and Coomassie blue staining.
80%; Purified by SDS-PAGE and Coomassie blue staining.
Form/Format
Supplied as a liquid in 50mM Tris, pH 7.5, 150mM NaCl, 0.1% 2-mercaptoethanol, 0.1mM EGTA, 1mM benzamidine, 0.2mM PMSF, 0.03% Brij-35 and 50% glycerol.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
SDS-Page
(SDS-PAGE and Coomassie Stain: Purity was assessed by|SDS-PAGE and Coomassie blue staining using 3ug of A1125-08.)
SDS-Page
(SDS-PAGE and Coomassie Stain: Purity was assessed by|SDS-PAGE and Coomassie blue staining using 3ug of A1125-08.)
Related Product Information for Akt1, unactivated recombinant protein
The serine/threonine kinase Akt1 has also been described as Rac-PKa or PKBa. Rac protein kinase family members feature a pleckstrin homology (PH) domain at the amino terminus and a protein-serine/threonine kinase catalytic domain at the carboxy terminus. The PH domain (amino acids 1-106) has been implicated in interactions with other proteins such as G-protein beta gamma subunits, as well as phosphoinositides. The kinase domain is located between residues 148-411. These enzymes are activated by diverse ligands such as PGDF, EGF and basic FG in NIH 3T3, Rat-1 or Swiss-3T3 cells. Akt1 is a protein of 480 amino acids and has been observed to show different migratory patterns on a western blot according to the state of phosphorylation of the protein. Phosphatase treatment has been shown to result in inactivation of the protein.
N-terminus His6-tagged recombinant full length human Akt1, expressed by baculovirus in Sf21 insect.
N-terminus His6-tagged recombinant full length human Akt1, expressed by baculovirus in Sf21 insect.
Product Categories/Family for Akt1, unactivated recombinant protein
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Product Notes
The Akt1, unactivated (Catalog #AAA636824) is a Recombinant Protein produced from Recombinant human and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Akt1, unactivated, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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