Rabbit anti-Human ENPP2 Polyclonal Antibody | anti-ENPP2 antibody

ENPP2, ID (K416) (Ectonucleotide Pyrophosphatase/Phosphodiesterase Family Member 2, E-NPP 2, Extracellular Lysophospholipase D, LysoPLD, Autotaxin, ATX, PDNP2) (MaxLight 405)

Cat. Num. AAA6474063

• Gene Names: ENPP2; ATX; NPP2; ATX-X; PDNP2; LysoPLD; AUTOTAXIN; PD-IALPHA
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: ENPP2; Polyclonal Antibody; ID (K416) (Ectonucleotide Pyrophosphatase/Phosphodiesterase Family Member 2; E-NPP 2; Extracellular Lysophospholipase D; LysoPLD; Autotaxin; ATX; PDNP2) (MaxLight 405); anti-ENPP2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human ENPP2.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight405.

• Applicable Applications for anti-ENPP2 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:50-1:100; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the center region of human ENPP2 (KLH).
• Conjugate: MaxLight405
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight405 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of anti-ENPP2 Antibody (Center K416) in Y79 cell line lysates (35ug/lane).ENPP2(arrow) was detected using the purified Pab.)

Western Blot (WB)

(Western blot analysis of anti-ENPP2 Antibody (Center K416) in mouse cerebellum tissue lysates (35ug/lane).ENPP2(arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(ENPP2 Antibody (Center K416) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ENPP2 Antibody (Center K416) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(ENPP2 Antibody (Center K416) flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-ENPP2 antibody

Antibodies; Enzymes; Phosphatase

References

1. Kawagoe H., Soma O., Goji J., Nishimura N., Narita M., Genomics 30:380-384(1995). 2. Nam S.W., Clair T., Kim Y.S., McMarlin A., Cancer Res. 61:6938-6944(2001). 3. The MGC Project Team Genome Res. 14:2121-2127(2004).

NCBI and Uniprot Product Information

• NCBI GI #: 91823602
• NCBI GeneID: 5168
• NCBI Accession #: NP_001035181
• NCBI GenBank Nucleotide #: NM_001040092
• UniProt Accession #: Q13822; Q13827; Q14555; Q15117; Q9UCQ8; Q9UCR0; Q9UCR1; Q9UCR2; Q9UCR3; Q9UCR4; A8UHA1; E9PHP7
• Molecular Weight: 101,968 Da
• NCBI Official Full Name: ectonucleotide pyrophosphatase/phosphodiesterase family member 2 isoform 2 preproprotein
• NCBI Official Synonym Full Names: ectonucleotide pyrophosphatase/phosphodiesterase 2
• NCBI Official Symbol: ENPP2
• NCBI Official Synonym Symbols: ATX; NPP2; ATX-X; PDNP2; LysoPLD; AUTOTAXIN; PD-IALPHA
• NCBI Protein Information: ectonucleotide pyrophosphatase/phosphodiesterase family member 2; E-NPP 2; autotaxin-t; extracellular lysophospholipase D; phosphodiesterase I/nucleotide pyrophosphatase 2; plasma lysophospholipase D
• UniProt Protein Name: Ectonucleotide pyrophosphatase/phosphodiesterase family member 2
• Protein Family: Ectonucleotide pyrophosphatase/phosphodiesterase family
• UniProt Gene Name: ENPP2
• UniProt Synonym Gene Names: ATX; PDNP2; E-NPP 2; LysoPLD
• UniProt Entry Name: ENPP2_HUMAN

Uniprot Description

ENPP2: Hydrolyzes lysophospholipids to produce lysophosphatidic acid (LPA) in extracellular fluids. Major substrate is lysophosphatidylcholine. Also can act on sphingosylphosphphorylcholine producing sphingosine-1-phosphate, a modulator of cell motility. Can hydrolyze, in vitro, bis-pNPP, to some extent pNP-TMP, and barely ATP. Involved in several motility- related processes such as angiogenesis and neurite outgrowth. Acts as an angiogenic factor by stimulating migration of smooth muscle cells and microtubule formation. Stimulates migration of melanoma cells, probably via a pertussis toxin-sensitive G protein. May have a role in induction of parturition. Possible involvement in cell proliferation and adipose tissue development. Tumor cell motility-stimulating factor. Belongs to the nucleotide pyrophosphatase/phosphodiesterase family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Lipid Metabolism - ether lipid; Phosphodiesterase; Secreted, signal peptide; Secreted; Motility/polarity/chemotaxis; EC 3.1.4.39

Chromosomal Location of Human Ortholog: 8q24.1

Cellular Component: extracellular space; integral to plasma membrane; plasma membrane

Molecular Function: nucleotide diphosphatase activity; phosphodiesterase I activity; nucleic acid binding; hydrolase activity; zinc ion binding; calcium ion binding; transcription factor binding; scavenger receptor activity; alkylglycerophosphoethanolamine phosphodiesterase activity; polysaccharide binding; lysophospholipase activity

Biological Process: G-protein coupled receptor protein signaling pathway; receptor-mediated endocytosis; phospholipid catabolic process; immune response; cell motility; chemotaxis; regulation of angiogenesis; phosphate metabolic process; regulation of cell migration