Testing Data
(P-peptide-+)
anti-Human, Mouse MLK1/2 Antibody | anti-MLK1/2 antibody
MLK1/2 (Phospho-Thr312/266) Antibody
Gene Names
MAP3K9; MLK1; MEKK9; PRKE1
Reactivity
Human, Mouse
Applications
Immunohistochemistry, ELISA
Purity
Affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Synonyms
MLK1/2; MLK1/2 (Phospho-Thr312/266) Antibody; EC 2.7.11.25; M3K9; mitogen-activated protein kinase kinase kinase 9; mixed-lineage protein kinase 1; PRKE1; anti-MLK1/2 antibody
Reactivity
Human, Mouse
Specificity
MLK1/2 (Phospho-Thr312/266) antibody detects endogenous levels of MLK1/2 only when phosphorylated at threonine 312/266.
Purity/Purification
Affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Sequence Length
1104
Applicable Applications for anti-MLK1/2 antibody
Immunohistochemistry (IHC), ELISA (EIA)
Application Notes
IHC: 1:50-1:100
ELISA: 1:5000
ELISA: 1:5000
Immunogen
The antiserum was produced against synthesized phosphopeptide derived from human MLK1/2 around the phosphorylation site of threonine 312/266 (A-G-TP-Y-A).
All Sites
Human: Thr312/266
Mouse: Thr305/266
Mouse: Thr305/266
Preparation and Storage
Store at-20 degree C for 1 year.
Testing Data
(P-peptide-+)
Testing Data
(P-peptide-+)
Immunohistochemistry (IHC)
(Immunohistochemistry analysis of paraffin-embedded human brain tissue using MLK1/2 (Phospho-Thr312/266) antibody. MLK1/2 (Phospho-Thr312/266) antibody reacts with epitope-specific phosphopeptide and corresponding non-phosphopeptide. The absorbance readings at 450 nM are shown in the ELISA figure.)
Immunohistochemistry (IHC)
(Immunohistochemistry analysis of paraffin-embedded human brain tissue using MLK1/2 (Phospho-Thr312/266) antibody. MLK1/2 (Phospho-Thr312/266) antibody reacts with epitope-specific phosphopeptide and corresponding non-phosphopeptide. The absorbance readings at 450 nM are shown in the ELISA figure.)
Product Categories/Family for anti-MLK1/2 antibody
References
Durkin J.T., Biochemistry 43:16348-16355(2004). The MGC Project Team; Genome Res. 14:2121-2127(2004). Dorow D.S., Nature 446:153-158(2007).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
123,373 Da
NCBI Official Full Name
mitogen-activated protein kinase kinase kinase 9 isoform 2
NCBI Official Synonym Full Names
mitogen-activated protein kinase kinase kinase 9
NCBI Official Symbol
MAP3K9
NCBI Official Synonym Symbols
MLK1; MEKK9; PRKE1
NCBI Protein Information
mitogen-activated protein kinase kinase kinase 9
UniProt Protein Name
Mitogen-activated protein kinase kinase kinase 9
UniProt Gene Name
MAP3K9
UniProt Synonym Gene Names
MLK1; PRKE1
Uniprot Description
Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. Plays an important role in the cascades of cellular responses evoked by changes in the environment. Once activated, acts as an upstream activator of the MKK/JNK signal transduction cascade through the phosphorylation of MAP2K4/MKK4 and MAP2K7/MKK7 which in turn activate the JNKs. The MKK/JNK signaling pathway regulates stress response via activator protein-1 (JUN) and GATA4 transcription factors. Plays also a role in mitochondrial death signaling pathway, including the release cytochrome c, leading to apoptosis.
Research Articles on MLK1/2
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Product Notes
The MLK1/2 map3k9 (Catalog #AAA856198) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The MLK1/2 (Phospho-Thr312/266) Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's MLK1/2 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), ELISA (EIA). IHC: 1:50-1:100 ELISA: 1:5000. Researchers should empirically determine the suitability of the MLK1/2 map3k9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MLK1/2, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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