ADARB1 / Double-Stranded RNA-Specific Editase 1 Recombinant Protein | ADARB1 recombinant protein

Human ADARB1 / Double-Stranded RNA-Specific Editase 1 Recombinant Protein

• Gene Names: ADARB1; RED1; ADAR2; DRABA2; DRADA2
• Applications: ELISA, Western Blot
• Purity: >95% by SDS-PAGE
• Synonyms: ADARB1 / Double-Stranded RNA-Specific Editase 1; Human ADARB1 / Double-Stranded RNA-Specific Editase 1 Recombinant Protein; Double-stranded RNA-specific editase 1; RNA-editing deaminase 1; RNA-editing enzyme 1; dsRNA adenosine deaminase; ADAR2; DRADA2; RED1; ADARB1 recombinant protein

• Host: E Coli
• Purity/Purification: >95% by SDS-PAGE
• Form/Format: Liquid; 50mM NaH2PO4, 500mM NaCl Buffer with 500mM Imidazole, 10% Glycerol (PH8.0)
• Concentration: Reconstitution Dependent (varies by lot)
• Sequence Length: 701

• Applicable Applications for ADARB1 recombinant protein: ELISA (EIA), Western Blot (WB), Antigen Protein (AP), Protein Array
• Species: Human
• Tag: His
• Subunit: Homodimer. Homodimerization is essential for its catalytic activity. Can form heterodimers with isoform 5 of ADAR/ADAR1.
• Entry Function: Catalyzes the hydrolytic deamination of adenosine to inosine in double-stranded RNA (dsRNA) referred to as A-to-I RNA editing. This may affect gene expression and function in a number of ways that include mRNA translation by changing codons and hence the amino acid sequence of proteins; pre-mRNA splicing by altering splice site recognition sequences; RNA stability by changing sequences involved in nuclease recognition; genetic stability in the case of RNA virus genomes by changing sequences during viral RNA replication; and RNA structure-dependent activities such as microRNA production or targeting or protein-RNA interactions. Can edit both viral and cellular RNAs and can edit RNAs at multiple sites (hyper-editing) or at specific sites (site-specific editing). Its cellular RNA substrates include: bladder cancer-associated protein (BLCAP), neurotransmitter receptors for glutamate (GRIA2 and GRIK2) and serotonin (HTR2C), GABA receptor (GABRA3) and potassium voltage-gated channel (KCNA1). Site-specific RNA editing of transcripts encoding these proteins results in amino acid substitutions which consequently alter their functional activities. Edits GRIA2 at both the Q/R and R/G sites efficiently but converts the adenosine in hotspot1 much less efficiently. Can exert a proviral effect towards human immunodeficiency virus type 1 (HIV-1) and enhances its replication via both an editing-dependent and editing-independent mechanism. The former involves editing of adenosines in the 5'UTR while the latter occurs via suppression of EIF2AK2/PKR activation and function. Can inhibit cell proliferation and migration and can stimulate exocytosis.
• Preparation and Storage: Store at -20 degree C. Avoid repeated freezing and thawing.

NCBI and Uniprot Product Information

• NCBI GI #: 4501919
• NCBI GeneID: 104
• NCBI Accession #: NP_001103.1
• NCBI GenBank Nucleotide #: NP_001103.1
• UniProt Accession #: P78563
• NCBI Official Full Name: double-stranded RNA-specific editase 1 isoform 1
• NCBI Official Synonym Full Names: adenosine deaminase RNA specific B1
• NCBI Official Symbol: ADARB1
• NCBI Official Synonym Symbols: RED1; ADAR2; DRABA2; DRADA2
• NCBI Protein Information: double-stranded RNA-specific editase 1
• UniProt Protein Name: Double-stranded RNA-specific editase 1
• Protein Family: Double-stranded RNA-specific editase
• UniProt Gene Name: ADARB1
• UniProt Synonym Gene Names: ADAR2; DRADA2; RED1
• UniProt Entry Name: RED1_HUMAN

NCBI Description

This gene encodes the enzyme responsible for pre-mRNA editing of the glutamate receptor subunit B by site-specific deamination of adenosines. Studies in rat found that this enzyme acted on its own pre-mRNA molecules to convert an AA dinucleotide to an AI dinucleotide which resulted in a new splice site. Alternative splicing of this gene results in several transcript variants, some of which have been characterized by the presence or absence of an ALU cassette insert and a short or long C-terminal region. [provided by RefSeq, Jul 2008]

Uniprot Description

RED1: Editing of the messenger RNAs for glutamate receptor (GluR) subunits by site-selective adenosine deamination. Edits both the GluR-B Q/R and R/G sites efficiently but converts the adenosine in hotspot1 much less efficiently. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: RNA-binding; Nucleolus; Hydrolase; EC 3.5.4.37

Chromosomal Location of Human Ortholog: 21q22.3

Cellular Component: nucleoplasm; cytoplasm; nucleolus; nucleus

Molecular Function: adenosine deaminase activity; mRNA binding; double-stranded RNA adenosine deaminase activity; protein binding; RNA binding; metal ion binding; double-stranded RNA binding

Biological Process: RNA processing; negative regulation of cell proliferation; base conversion or substitution editing; positive regulation of viral genome replication; regulation of cell cycle; mRNA modification; innate immune response; gene expression; mRNA processing; defense response to virus; adenosine to inosine editing; negative regulation of cell migration