Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human colon stained with CEA MAb (CEA31))
Mouse Carcinoembryonic Antigen Monoclonal Antibody | anti-CEA antibody
Carcinoembryonic Antigen (Carcinoembryonic Antigen-related Cell Adhesion Molecule 5, CEACAM5, CEA, CD66, Biliary Glycoprotein (BGP-1)) (APC)
Gene Names
CEACAM5; CEA; CD66e
Reactivity
Human, Monkey
Applications
Flow Cytometry, Functional Assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Purified by Protein A/G Affinity Chromatography.
Synonyms
Carcinoembryonic Antigen; Monoclonal Antibody; Carcinoembryonic Antigen (Carcinoembryonic Antigen-related Cell Adhesion Molecule 5; CEACAM5; CEA; CD66; Biliary Glycoprotein (BGP-1)) (APC); Carcinoembryonic Antigen-related Cell Adhesion Molecule 5; Biliary Glycoprotein (BGP-1); anti-CEA antibody
Host
Mouse
Reactivity
Human, Monkey
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
CEA31
Specificity
Recognizes human Carcinoembryonic Antigen. Species Crossreactivity: monkey. Others not known.
Purity/Purification
Purified by Protein A/G Affinity Chromatography.
Form/Format
Supplied as a liquid in 10mM PBS. No preservative added. BSA-Free. Labeled with Allophycocyanin (APC).
Applicable Applications for anti-CEA antibody
Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC) Frozen/Paraffin, Immunoprecipitation (IP), Western Blot (WB)
Application Notes
FC/FACS: 0.5-1ug/million cells
IHC-F/P: 1-2ug/ml for 30 min at RT
IP: 1-2ug/500ug protein
IF: 1-2ug/ml
WB: 0.25-0.5ug/ml
Applications are based on unconjugated antibody.
IHC-F/P: 1-2ug/ml for 30 min at RT
IP: 1-2ug/500ug protein
IF: 1-2ug/ml
WB: 0.25-0.5ug/ml
Applications are based on unconjugated antibody.
Immunogen
Human colon carcinoma extract. Cellular Localization: Cytoplasmic and luminal surface
Conjugate
APC
Positive Control
MCF7 or 293T cells. Colon carcinoma
Special Handling
Light sensitive
Preparation and Storage
May be stored at 4 degree C before opening. DO NOT FREEZE! Stable at 4 degree C as an undiluted liquid. Dilute only prior to immediate use. Stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer. APC is light sensitive.
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human colon stained with CEA MAb (CEA31))
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human colon stained with CEA MAb (CEA31))
Product Categories/Family for anti-CEA antibody
References
1. Muraro R, et. al. Cancer Research, 1985, 45:5769-80. 2. Siler K, et. al. Biotechnology Therapeutics, 1993, 4(3-4):163-81. 3. Robbins PF, et. al. International Journal of Cancer, 1993, 53(6):892-7.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
76,795 Da
NCBI Official Full Name
Carcinoembryonic antigen-related cell adhesion molecule 5
NCBI Official Synonym Full Names
carcinoembryonic antigen-related cell adhesion molecule 5
NCBI Official Symbol
CEACAM5
NCBI Official Synonym Symbols
CEA; CD66e
NCBI Protein Information
carcinoembryonic antigen-related cell adhesion molecule 5; meconium antigen 100
UniProt Protein Name
Carcinoembryonic antigen-related cell adhesion molecule 5
Protein Family
UniProt Gene Name
CEACAM5
UniProt Synonym Gene Names
CEA; CEA
UniProt Entry Name
CEAM5_HUMAN
NCBI Description
Members of the CEACAM subfamily, including CEACAM5, belong to the CEA gene family. For general information on the CEA gene family, see CEACAM1 (MIM 109770).[supplied by OMIM, Oct 2009]
Uniprot Description
CEACAM5: Cell surface glycoprotein that plays a role in cell adhesion and in intracellular signaling. Receptor for E.coli Dr adhesins. Homodimer. Binding of E.coli Dr adhesins leads to dissociation of the homodimer. Found in adenocarcinomas of endodermally derived digestive system epithelium and fetal colon. Belongs to the immunoglobulin superfamily. CEA family.
Protein type: Membrane protein, integral; Immunoglobulin superfamily; Membrane protein, GPI anchor
Chromosomal Location of Human Ortholog: 19q13.1-q13.2
Cellular Component: basolateral plasma membrane; integral to plasma membrane
Molecular Function: identical protein binding; protein homodimerization activity
Biological Process: homotypic cell-cell adhesion; negative regulation of apoptosis
Research Articles on CEA
Similar Products
Product Notes
The CEA ceacam5 (Catalog #AAA6122592) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Carcinoembryonic Antigen (Carcinoembryonic Antigen-related Cell Adhesion Molecule 5, CEACAM5, CEA, CD66, Biliary Glycoprotein (BGP-1)) (APC) reacts with Human, Monkey and may cross-react with other species as described in the data sheet. AAA Biotech's Carcinoembryonic Antigen can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC) Frozen/Paraffin, Immunoprecipitation (IP), Western Blot (WB). FC/FACS: 0.5-1ug/million cells IHC-F/P: 1-2ug/ml for 30 min at RT IP: 1-2ug/500ug protein IF: 1-2ug/ml WB: 0.25-0.5ug/ml Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the CEA ceacam5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Carcinoembryonic Antigen, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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