Mouse anti-Human FAK Monoclonal Antibody | anti-FAK antibody

Anti-FAK Antibody

Cat. Num. AAA9231138

• Gene Names: PTK2; FAK; FADK; FAK1; FRNK; PPP1R71; p125FAK; pp125FAK
• Reactivity: Human
• Applications: Western Blot, Immunofluorescence, Immunoprecipitation
• Synonyms: FAK; Monoclonal Antibody; Anti-FAK Antibody; FADK 1; FADK; FAK 1; FAK related non kinase polypeptide; FAK1; FAK1_HUMAN; Focal adhesion kinase 1; Focal adhesion Kinase; Focal adhesion kinase isoform FAK Del33; Focal adhesion kinase related nonkinase; FRNK; p125FAK; pp125FAK; PPP1R71; Protein phosphatase 1 regulatory subunit 71; Protein tyrosine kinase 2; Protein Tyrosine Kinase Cytoplasmic; Protein-tyrosine kinase 2; PTK 2; PTK2; PTK2 protein tyrosine kinase 2; PTK2 protein tyrosine kinase 2.; anti-FAK antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Form/Format: PBS (pH 7.4) containing with 0.03% Proclin300 and 50% glycerol.
• Sequence Length: 1065

• Applicable Applications for anti-FAK antibody: Western Blot (WB), Immunofluorescence (IF), Immunoprecipitation (IP)
• Application Notes: WB: 1:1000; ICC: 1:200
• Cellular Location: Cell junction, focal adhesion. Cell membrane; Peripheral membrane protein; Cytoplasmic side Cytoplasm, cell cortex. Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Nucleus. Note=Constituent of focal adhesions Detected at microtubules
• Tissue Location: Detected in B and T-lymphocytes. Isoform 1 and isoform 6 are detected in lung fibroblasts (at protein level) Ubiquitous.
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt

Western Blot (WB)

(Western blot detection of FAK in COS7,HUVEC,A549 and Hela cell lysates using FAK mouse mAb (1:1000 diluted).Predicted band size: 125KDa.Observed band size:125KDa.)

Immunocytochemistry (IC)

(Immunofluorescent analysis of Hela cells fixed fixed by anhydrous methanol at -20 degree C and using FAK mouse mAb (dilution 1:200).)

Immunoprecipitation (IP)

(Immunoprecipitation analysis of Hela cell lysates using FAK mouse mAb.)

Related Product Information for anti-FAK antibody

Non-receptor protein-tyrosine kinase that plays an essential role in regulating cell migration, adhesion, spreading, reorganization of the actin cytoskeleton, formation and disassembly of focal adhesions and cell protrusions, cell cycle progression, cell proliferation and apoptosis. Required for early embryonic development and placenta development. Required for embryonic angiogenesis, normal cardiomyocyte migration and proliferation, and normal heart development. Regulates axon growth and neuronal cell migration, axon branching and synapse formation; required for normal development of the nervous system. Plays a role in osteogenesis and differentiation of osteoblasts. Functions in integrin signal transduction, but also in signaling downstream of numerous growth factor receptors, G-protein coupled receptors (GPCR), EPHA2, netrin receptors and LDL receptors. Forms multisubunit signaling complexes with SRC and SRC family members upon activation; this leads to the phosphorylation of additional tyrosine residues, creating binding sites for scaffold proteins, effectors and substrates. Regulates numerous signaling pathways. Promotes activation of phosphatidylinositol 3-kinase and the AKT1 signaling cascade. Promotes activation of MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling cascade. Promotes localized and transient activation of guanine nucleotide exchange factors (GEFs) and GTPase-activating proteins (GAPs), and thereby modulates the activity of Rho family GTPases. Signaling via CAS family members mediates activation of RAC1. Recruits the ubiquitin ligase MDM2 to P53/TP53 in the nucleus, and thereby regulates P53/TP53 activity, P53/TP53 ubiquitination and proteasomal degradation. Phosphorylates SRC; this increases SRC kinase activity. Phosphorylates ACTN1, ARHGEF7, GRB7, RET and WASL. Promotes phosphorylation of PXN and STAT1; most likely PXN and STAT1 are phosphorylated by a SRC family kinase that is recruited to autophosphorylated PTK2/FAK1, rather than by PTK2/FAK1 itself. Promotes phosphorylation of BCAR1; GIT2 and SHC1; this requires both SRC and PTK2/FAK1. Promotes phosphorylation of BMX and PIK3R1. Isoform 6 (FRNK) does not contain a kinase domain and inhibits PTK2/FAK1 phosphorylation and signaling. Its enhanced expression can attenuate the nuclear accumulation of LPXN and limit its ability to enhance serum response factor (SRF)-dependent gene transcription.

NCBI and Uniprot Product Information

• NCBI GI #: 313851044
• NCBI GeneID: 5747
• NCBI Accession #: NP_001186578.1
• NCBI GenBank Nucleotide #: NM_153831.2
• UniProt Accession #: Q05397; Q14291; Q8IYN9; Q9UD85; B4E2N6; F5H4S4; J3QT16
• Molecular Weight: 125 KDa
• NCBI Official Full Name: focal adhesion kinase 1 isoform c
• NCBI Official Synonym Full Names: protein tyrosine kinase 2
• NCBI Official Symbol: PTK2
• NCBI Official Synonym Symbols: FAK; FADK; FAK1; FRNK; PPP1R71; p125FAK; pp125FAK
• NCBI Protein Information: focal adhesion kinase 1
• UniProt Protein Name: Focal adhesion kinase 1
• UniProt Gene Name: PTK2
• UniProt Synonym Gene Names: FAK; FAK1; FADK 1; FRNK; PPP1R71

NCBI Description

This gene encodes a cytoplasmic protein tyrosine kinase which is found concentrated in the focal adhesions that form between cells growing in the presence of extracellular matrix constituents. The encoded protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies. Activation of this gene may be an important early step in cell growth and intracellular signal transduction pathways triggered in response to certain neural peptides or to cell interactions with the extracellular matrix. Several transcript variants encoding different isoforms have been found for this gene, but the full-length natures of only four of them have been determined. [provided by RefSeq, Oct 2015]

Uniprot Description

FAK: a tyrosine kinase of the FAK family required for cell migration and contact-dependent survival signaling. Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy. Downstream of integrins and Src, upstream of Ras/MAPK. Localizes to focal adhesions that form between cells growing in the presence of extracellular matrix constituents. Interacts with CAS family members and with GIT1, SORBS1 and BCAR3. Interacts with Shb. Required for full Ras transformation of fibroblasts. Increased expression in breast and other cancers, related to chromosome 8q amplification. Overexpression and activation associated with increased migration, invasion and progression of ovarian cancer, and with progression in hepatocellular carcinoma, thyroid cancer, and acute myelogenous leukemia. siRNA increases chemosensitivity of pancreatic adenocarcinoma xenografts. Inhibitor: ISI15421 (antisense). Four splice-variant isoforms have been observed.

Protein type: EC 2.7.10.2; Fak family; Kinase, protein; Protein kinase, TK; Protein kinase, tyrosine (non-receptor); TK group

Chromosomal Location of Human Ortholog: 8q24.3

Cellular Component: cytoplasm; cytoskeleton; cytosol; extrinsic to internal side of plasma membrane; focal adhesion; nucleus; plasma membrane; stress fiber

Molecular Function: actin binding; JUN kinase binding; non-membrane spanning protein tyrosine kinase activity; protein binding; protein kinase activity; protein kinase binding; protein-tyrosine kinase activity; Ras guanyl-nucleotide exchange factor activity; receptor binding; SH2 domain binding

Biological Process: angiogenesis; axon guidance; cell motility involved in cell locomotion; cell structure disassembly during apoptosis; embryonic development; ephrin receptor signaling pathway; epidermal growth factor receptor signaling pathway; establishment of cell polarity; heart morphogenesis; innate immune response; integrin-mediated signaling pathway; MAPKKK cascade; negative regulation of apoptosis; negative regulation of cell-cell adhesion; peptidyl-tyrosine phosphorylation; placenta development; positive regulation of cell migration; positive regulation of cell proliferation; positive regulation of phosphoinositide 3-kinase activity; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of protein amino acid phosphorylation; positive regulation of protein kinase activity; positive regulation of protein kinase B signaling cascade; protein amino acid autophosphorylation; regulation of cell adhesion mediated by integrin; regulation of cell proliferation; regulation of cell shape; regulation of cytoskeleton organization and biogenesis; regulation of focal adhesion formation; regulation of GTPase activity; regulation of osteoblast differentiation; regulation of protein amino acid phosphorylation; transforming growth factor beta receptor signaling pathway; vascular endothelial growth factor receptor signaling pathway