Mouse Double-stranded RNA-specific adenosine deaminase ELISA Kit | Adar elisa kit

Mouse Double-stranded RNA-specific adenosine deaminase ELISA Kit

Cat. Num. AAA2887879

• Gene Names: Adar; Adar1; mZaADAR; AV242451; Adar1p110; Adar1p150
• Reactivity: Mouse
• Synonyms: Double-stranded RNA-specific adenosine deaminase; Mouse Double-stranded RNA-specific adenosine deaminase ELISA Kit; DRADA; RNA adenosine deaminase 1; Adar; 3.5.4.37; Adar elisa kit

• Reactivity: Mouse
• Specificity: Recombinant and natural Mouse Double-stranded RNA-specific adenosine deaminase,DRADA
• Sequence Length: 1152

• Samples: Serum, plasma, tissue homogenates, cell culture supernates or other biological fluids
• Detection Range: 0.156-10 ng/mL
• Sensitivity: <0.083 ng/mL
• Intra-assay Precision: Intra-Assay CV: <=4.8%
• Inter-assay Precision: Inter-Assay CV: <=6.7%
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Adar elisa kit

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 226371679
• NCBI GeneID: 56417
• NCBI Accession #: NP_062629.3
• NCBI GenBank Nucleotide #: NM_019655.3
• Molecular Weight: 102,580 Da
• NCBI Official Full Name: double-stranded RNA-specific adenosine deaminase isoform 1
• NCBI Official Synonym Full Names: adenosine deaminase, RNA-specific
• NCBI Official Symbol: Adar
• NCBI Official Synonym Symbols: Adar1; mZaADAR; AV242451; Adar1p110; Adar1p150
• NCBI Protein Information: double-stranded RNA-specific adenosine deaminase
• UniProt Protein Name: Double-stranded RNA-specific adenosine deaminase
• Protein Family: Double-stranded RNA-specific editase
• UniProt Gene Name: Adar
• UniProt Synonym Gene Names: DRADA
• UniProt Entry Name: DSRAD_MOUSE

Uniprot Description

ADAR: Converts multiple adenosines to inosines and creates I/U mismatched base pairs in double-helical RNA substrates without apparent sequence specificity. Has been found to modify more frequently adenosines in AU-rich regions, probably due to the relative ease of melting A/U base pairs as compared to G/C pairs. Functions to modify viral RNA genomes and may be responsible for hypermutation of certain negative-stranded viruses. Edits the messenger RNAs for glutamate receptor (GLUR) subunits by site- selective adenosine deamination. Produces low-level editing at the GLUR-B Q/R site, but edits efficiently at the R/G site and HOTSPOT1. Binds to short interfering RNAs (siRNA) without editing them and suppresses siRNA-mediated RNA interference. Binds to ILF3/NF90 and up-regulates ILF3-mediated gene expression. Isoform 1 is induced by interferon alpha. Isoform 5 is constitutively expressed. Homodimer. Isoform 1 interacts with ILF2/NF45 and ILF3/NF90. 5 isoforms of the human protein are produced by alternative promoter.

Protein type: RNA processing; Hydrolase; Nucleolus; EC 3.5.4.37; RNA-binding

Cellular Component: cytoplasm; membrane; nuclear speck; nucleolus; nucleoplasm; nucleus

Molecular Function: double-stranded RNA adenosine deaminase activity; left-handed Z-DNA binding; protein binding

Biological Process: adenosine to inosine editing; base conversion or substitution editing; defense response to virus; erythrocyte differentiation; hemopoietic progenitor cell differentiation; in utero embryonic development; miRNA-mediated gene silencing, miRNA loading onto RISC; miRNA-mediated gene silencing, production of miRNAs; negative regulation of apoptosis; negative regulation of viral genome replication; osteoblast differentiation; positive regulation of viral genome replication; pre-microRNA processing; protein export from nucleus; protein import into nucleus; response to virus; somatic diversification of immune receptors via somatic mutation