Mouse TNF receptor-associated factor 6 ELISA Kit | Traf6 elisa kit

Mouse TNF receptor-associated factor 6 ELISA Kit

Cat. Num. AAA2882029

• Gene Names: Traf6; AI851288; 2310003F17Rik; C630032O20Rik
• Reactivity: Mouse
• Synonyms: TNF receptor-associated factor 6; Mouse TNF receptor-associated factor 6 ELISA Kit; E3 ubiquitin-protein ligase TRAF6; Traf6; Traf6 elisa kit

• Reactivity: Mouse
• Specificity: Recombinant and natural Mouse TNF receptor-associated factor 6
• Sequence Length: 530

• Samples: Serum, plasma, tissue homogenates, cell culture supernates or other biological fluids
• Detection Range: 0.156-10 ng/mL
• Sensitivity: <0.082 ng/mL
• Intra-assay Precision: Intra-Assay CV: <=4.4%
• Inter-assay Precision: Inter-Assay CV: <=6.7%
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Traf6 elisa kit

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 741866086
• NCBI GeneID: 22034
• NCBI Accession #: NP_001290202.1
• NCBI GenBank Nucleotide #: NM_001303273.1
• UniProt Accession #: P70196; Q6P9M0; Q8BLV2
• Molecular Weight: 18,961 Da
• NCBI Official Full Name: TNF receptor-associated factor 6
• NCBI Official Synonym Full Names: TNF receptor-associated factor 6
• NCBI Official Symbol: Traf6
• NCBI Official Synonym Symbols: AI851288; 2310003F17Rik; C630032O20Rik
• NCBI Protein Information: TNF receptor-associated factor 6
• UniProt Protein Name: TNF receptor-associated factor 6
• Protein Family: TNF receptor-associated factor
• UniProt Gene Name: Traf6
• UniProt Entry Name: TRAF6_MOUSE

NCBI Description

This gene encodes a member of the TNF receptor associated factor (TRAF) family of adaptor proteins that mediate signaling events from members of the TNF receptor and Toll/IL-1 receptor families to activate transcription factors such as NF-kappa-B and AP-1. The product of this gene is essential for perinatal and postnatal survival. Mice deficient in this protein exhibit osteopetrosis and defective in development of epidermal appendixes, normal B cell differentiation, lymph node organogenesis, interleukin-1 signaling, lipopolysaccharide signaling and neural tube closure. This protein possesses ubiquitin ligase activity. Alternate splicing of this gene results in multiple transcript variants. [provided by RefSeq, Dec 2014]

Uniprot Description

TRAF6: E3 ubiquitin ligase that, together with UBE2N and UBE2V1, mediates the synthesis of 'Lys-63'-linked-polyubiquitin chains conjugated to proteins, such as IKBKG, AKT1 and AKT2. Also mediates ubiquitination of free/unanchored polyubiquitin chain that leads to MAP3K7 activation. Leads to the activation of NF- kappa-B and JUN. May be essential for the formation of functional osteoclasts. Seems to also play a role in dendritic cells (DCs) maturation and/or activation. Represses c-Myb-mediated transactivation, in B-lymphocytes. Adapter protein that seems to play a role in signal transduction initiated via TNF receptor, IL- 1 receptor and IL-17 receptor. Regulates osteoclast differentiation by mediating the activation of adapter protein complex 1 (AP-1) and NF-kappa-B, in response to RANK-L stimulation. Homotrimer. Homooligomer. N-terminal region is dimeric while C-terminal region is trimeric; maybe providing a mode of oligomerization. Binds to TNFRSF5/CD40 and TNFRSF11A/RANK. Associates with NGFR, TNFRSF17, IRAK1, IRAK2, IRAK3, IRAK4, RIPK2, MAP3K1, MAP3K5, MAP3K14, CSK, TRAF, TRAF-interacting protein TRIP and TNF receptor associated protein TDP2. Interacts with IL17R. Interacts with SQSTM1 bridging NTRK1 and NGFR. Forms a ternary complex with SQSTM1 and PRKCZ. Interacts with PELI1, PELI2 and PELI3. Binds UBE2V1. Interacts with MAVS/IPS1. Interacts with TAX1BP1. Interacts with IL1RL1. Interacts with TRAFD1. Interacts with ZNF675. Interacts with AJUBA. Interacts with TICAM1 and TICAM2. Interacts with ZFAND5. Interacts with ARRB1 and ARRB2. Interacts with MAP3K7 and TAB1/MAP3K7IP1; during IL-1 signaling. Interacts with UBE2N. Interacts with TGFBR1, HDAC1 and RANGAP1. Interacts with AKT1, AKT2 and AKT3. Interacts (via TRAF domains) with NUMBL (via C-terminal). Interacts (via TRAF domains) with WDR34 (via WD domains). Interacts with RBCK1. Interacts with TRAF3IP2. Interacts with LIMD1 (via LIM domains). Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Belongs to the TNF receptor-associated factor family. A subfamily.

Protein type: Ubiquitin ligase; EC 6.3.2.-; Ligase; Ubiquitin conjugating system

Cellular Component: cytoplasm; cytosol; internal side of plasma membrane; lipid particle; mitochondrion; nucleolus; nucleus; perinuclear region of cytoplasm; plasma membrane; protein complex

Molecular Function: coenzyme F420-0 gamma-glutamyl ligase activity; coenzyme F420-2 alpha-glutamyl ligase activity; histone deacetylase binding; identical protein binding; ligase activity; metal ion binding; mitogen-activated protein kinase kinase kinase binding; protein binding; protein kinase B binding; protein kinase binding; protein N-terminus binding; ribosomal S6-glutamic acid ligase activity; signal transducer activity; thioesterase binding; tumor necrosis factor receptor binding; ubiquitin conjugating enzyme binding; ubiquitin protein ligase binding; ubiquitin-protein ligase activity; UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity; zinc ion binding

Biological Process: activation of NF-kappaB transcription factor; activation of NF-kappaB-inducing kinase; activation of protein kinase activity; antigen processing and presentation of exogenous peptide antigen via MHC class II; bone remodeling; bone resorption; cell development; cytokine and chemokine mediated signaling pathway; I-kappaB kinase/NF-kappaB cascade; immune response; immune system process; JNK cascade; myeloid dendritic cell differentiation; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; neural tube closure; odontogenesis of dentine-containing teeth; organ morphogenesis; ossification; osteoclast differentiation; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of interleukin-12 biosynthetic process; positive regulation of interleukin-2 production; positive regulation of interleukin-6 biosynthetic process; positive regulation of JNK activity; positive regulation of lipopolysaccharide-mediated signaling pathway; positive regulation of osteoclast differentiation; positive regulation of smooth muscle cell proliferation; positive regulation of T cell cytokine production; positive regulation of T cell proliferation; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; protein autoubiquitination; protein complex assembly; protein polyubiquitination; protein ubiquitination; regulation of apoptosis; regulation of immunoglobulin secretion; signal transduction; T cell receptor signaling pathway; T-helper 1 type immune response