Typical Testing Data/Standard Curve (for reference only)
Rat Retinoic acid receptor RXR-alpha ELISA Kit | Rxra elisa kit
Rat Retinoic acid receptor RXR-alpha ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
displays 9-cis retinoic acid-dependent binding to transcription factor GATA-4 and corepressor FOG-2; regulates cardiac gene expression [RGD, Feb 2006]
Uniprot Description
RXRA: Receptor for retinoic acid. Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes. The RAR/RXR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. The high affinity ligand for RXRs is 9-cis retinoic acid. RXRA serves as a common heterodimeric partner for a number of nuclear receptors. The RXR/RAR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. In the absence of ligand, the RXR-RAR heterodimers associate with a multiprotein complex containing transcription corepressors that induce histone acetylation, chromatin condensation and transcriptional suppression. On ligand binding, the corepressors dissociate from the receptors and associate with the coactivators leading to transcriptional activation. The RXRA/PPARA heterodimer is required for PPARA transcriptional activity on fatty acid oxidation genes such as ACOX1 and the P450 system genes. Homodimer. Heterodimer with RARA; required for ligand- dependent retinoic acid receptor transcriptional activity. Heterodimer with PPARA (via the leucine-like zipper in the LBD); the interaction is required for PPARA transcriptional activity. Also heterodimerizes with PPARG. Interacts with NCOA3 and NCOA6 coactivators. Interacts with FAM120B. Interacts with PELP1, SENP6, SFPQ, DNTTIP2 and RNF8. Interacts (via the DNA binding domain) with HCV core protein; the interaction enhances the transcriptional activities of the RXRA/RARA and the RXRA/PPARA heterodimers. Interacts with PRMT2. Interacts with ASXL1 and NCOA1. Highly expressed in liver, also found in lung, kidney and heart. Belongs to the nuclear hormone receptor family. NR2 subfamily.
Protein type: Nuclear receptor; DNA-binding; Motility/polarity/chemotaxis
Cellular Component: axon; nuclear chromatin; nucleus; receptor complex
Molecular Function: chromatin DNA binding; DNA binding; double-stranded DNA binding; enzyme binding; ligand-dependent nuclear receptor activity; protein binding; protein complex binding; protein domain specific binding; protein heterodimerization activity; retinoic acid receptor binding; retinoid-X receptor activity; sequence-specific DNA binding; steroid hormone receptor activity; transcription factor activity; transcription factor binding; vitamin D receptor binding; zinc ion binding
Biological Process: aging; axon regeneration; camera-type eye development; cardiac muscle cell proliferation; cellular response to insulin stimulus; embryo implantation; female pregnancy; heart development; in utero embryonic development; inflammatory response; liver development; maternal placenta development; midgut development; negative regulation of cell proliferation; negative regulation of transcription from RNA polymerase II promoter; placenta development; positive regulation of apoptosis; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; positive regulation of translational initiation by iron; protein homotetramerization; regulation of myelination; regulation of transcription from RNA polymerase II promoter; regulation of transcription, DNA-dependent; response to ethanol; response to glucocorticoid stimulus; response to nutrient; response to organic cyclic substance; response to retinoic acid; response to selenium ion; response to vitamin A; response to vitamin D; retinoic acid receptor signaling pathway; steroid hormone mediated signaling; transcription, DNA-dependent; ventricular cardiac muscle cell differentiation; ventricular cardiac muscle morphogenesis; virus-host interaction
Research Articles on Rxra
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Product Notes
The Rat Rxra rxra (Catalog #AAA288114) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA288114 ELISA Kit recognizes Rat Rxra. It is sometimes possible for the material contained within the vial of "Retinoic acid receptor RXR-alpha, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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