Typical Testing Data/Standard Curve (for reference only)
Rat Urokinase-type plasminogen activator ELISA Kit | Plau elisa kit
Rat Urokinase-type plasminogen activator ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
displays a post-transcriptional increase in enzyme levels in chemically induced mammary carcinoma; may play a role in tumor invasion and metastasis [RGD, Feb 2006]
Uniprot Description
uPA: Specifically cleave the zymogen plasminogen to form the active enzyme plasmin. Defects in PLAU are the cause of Quebec platelet disorder (QPD). QPD is an autosomal dominant bleeding disorder due to a gain-of-function defect in fibrinolysis. Although affected individuals do not exhibit systemic fibrinolysis, they show delayed onset bleeding after challenge, such as surgery. The hallmark of the disorder is markedly increased PLAU levels within platelets, which causes intraplatelet plasmin generation and secondary degradation of alpha-granule proteins. Belongs to the peptidase S1 family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 3.4.21.73; Protease; Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide
Cellular Component: cell surface; extracellular space; focal adhesion; membrane
Molecular Function: kinase activity; peptidase activity; serine-type endopeptidase activity
Biological Process: angiogenesis; embryo implantation; fibrinolysis; phosphorylation; positive regulation of cell proliferation; positive regulation of smooth muscle cell migration; proteolysis; regulation of cell adhesion mediated by integrin; regulation of cell proliferation; regulation of receptor activity; regulation of smooth muscle cell migration; response to activity; response to hyperoxia; response to hypoxia; skeletal muscle regeneration; smooth muscle cell migration; spermatogenesis; wound healing
Research Articles on Plau
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Product Notes
The Rat Plau plau (Catalog #AAA2886195) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2886195 ELISA Kit recognizes Rat Plau. It is sometimes possible for the material contained within the vial of "Urokinase-type plasminogen activator, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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