Typical Testing Data/Standard Curve (for reference only)
Canine Von Willebrand Factor cleaving protease ELISA Kit | ADAMTS13/vWF-cp elisa kit
Canine Von Willebrand Factor cleaving protease ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: ADAMTS13 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-ADAMTS13 antibody and an ADAMTS13-HRP conjugate. The assay sample and buffer are incubated together with ADAMTS13-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ADAMTS13 concentration since ADAMTS13 from samples and ADAMTS13-HRP conjugate compete for the anti-ADAMTS13 antibody binding site. Since the number of sites is limited, as more sites are occupied by ADAMTS13 from the sample, fewer sites are left to bind ADAMTS13-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ADAMTS13 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of a family of proteins containing several distinct regions, including a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 (TS) motif. The enzyme encoded by this gene specifically cleaves von Willebrand Factor (vWF). Defects in this gene are associated with thrombotic thrombocytopenic purpura. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]
Uniprot Description
ADAMTS13: Cleaves the vWF multimers in plasma into smaller forms. Defects in ADAMTS13 are the cause of thrombotic thrombocytopenic purpura congenital (TTP); also known as Upshaw-Schulman syndrome (USS). A hematologic disease characterized by hemolytic anemia with fragmentation of erythrocytes, thrombocytopenia, diffuse and non-focal neurologic findings, decreased renal function and fever. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Motility/polarity/chemotaxis; Protease; Secreted; Calcium-binding; EC 3.4.24.87; Extracellular matrix; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 9q34
Cellular Component: proteinaceous extracellular matrix; extracellular space; cell surface; endoplasmic reticulum lumen
Molecular Function: integrin binding; protein binding; zinc ion binding; metallopeptidase activity; metalloendopeptidase activity; calcium ion binding
Biological Process: integrin-mediated signaling pathway; platelet activation; protein amino acid O-linked glycosylation; cellular protein metabolic process; glycoprotein metabolic process; peptide catabolic process; response to toxin; cell-matrix adhesion; protein processing; proteolysis; post-translational protein modification
Disease: Thrombotic Thrombocytopenic Purpura, Congenital
Research Articles on ADAMTS13/vWF-cp
Similar Products
Product Notes
The Canine ADAMTS13/vWF-cp adamts13 (Catalog #AAA735022) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA735022 ELISA Kit recognizes Canine ADAMTS13/vWF-cp. It is sometimes possible for the material contained within the vial of "Von Willebrand Factor cleaving protease, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
