Porcine Von Willebrand Factor cleaving protease ELISA Kit | vWF-cp elisa kit

Porcine Von Willebrand Factor cleaving protease ELISA Kit

Cat. Num. AAA741687

• Gene Names: ADAMTS13; VWFCP; C9orf8; vWF-CP; ADAM-TS13; ADAMTS-13
• Reactivity: Porcine
• Synonyms: Von Willebrand Factor cleaving protease; Porcine Von Willebrand Factor cleaving protease ELISA Kit; vWF-cp elisa kit

• Reactivity: Porcine

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Sandwich
• Sensitivity: 0.1 ng/mL.
• Intended Uses: This ADAMTS13/vWF-cp ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Porcine ADAMTS13/vWF-cp. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for vWF-cp elisa kit

Principle of the Assay: ADAMTS13/vWF-cp ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for ADAMTS13/vWF-cp. Standards or samples are then added to the microtiter plate wells and ADAMTS13/vWF-cp if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of ADAMTS13/vWF-cp present in the sample, a standardized preparation of horseradish peroxidase (HRP) -conjugated polyclonal antibody, specific for ADAMTS13/vWF-cp are added to each well to "sandwich" the ADAMTS13/vWF-cp immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain ADAMTS13/vWF-cp and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ADAMTS13/vWF-cp concentration in each sample is interpolated from this standard curve.

Product Categories/Family for vWF-cp elisa kit

Cardiovascular

NCBI and Uniprot Product Information

• NCBI GI #: 73695936
• NCBI GeneID: 11093
• NCBI Accession #: NP_620596.2
• NCBI GenBank Nucleotide #: NM_139027.4
• Molecular Weight: 39,864 Da
• NCBI Official Full Name: A disintegrin and metalloproteinase with thrombospondin motifs 13 isoform 2 preproprotein
• NCBI Official Synonym Full Names: ADAM metallopeptidase with thrombospondin type 1 motif, 13
• NCBI Official Symbol: ADAMTS13
• NCBI Official Synonym Symbols: VWFCP; C9orf8; vWF-CP; ADAM-TS13; ADAMTS-13
• NCBI Protein Information: A disintegrin and metalloproteinase with thrombospondin motifs 13; vWF-cleaving protease; von Willebrand factor-cleaving protease; a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 13
• UniProt Protein Name: A disintegrin and metalloproteinase with thrombospondin motifs 13
• UniProt Gene Name: ADAMTS13
• UniProt Synonym Gene Names: C9orf8; UNQ6102/PRO20085; ADAM-TS 13; ADAM-TS13; ADAMTS-13; vWF-CP; vWF-cleaving protease
• UniProt Entry Name: ATS13_HUMAN

NCBI Description

This gene encodes a member of a family of proteins containing several distinct regions, including a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 (TS) motif. The enzyme encoded by this gene specifically cleaves von Willebrand Factor (vWF). Defects in this gene are associated with thrombotic thrombocytopenic purpura. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]

Uniprot Description

ADAMTS13: Cleaves the vWF multimers in plasma into smaller forms. Defects in ADAMTS13 are the cause of thrombotic thrombocytopenic purpura congenital (TTP); also known as Upshaw-Schulman syndrome (USS). A hematologic disease characterized by hemolytic anemia with fragmentation of erythrocytes, thrombocytopenia, diffuse and non-focal neurologic findings, decreased renal function and fever. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Extracellular matrix; Motility/polarity/chemotaxis; Secreted; EC 3.4.24.87; Protease; Secreted, signal peptide; Calcium-binding

Chromosomal Location of Human Ortholog: 9q34

Cellular Component: extracellular space; proteinaceous extracellular matrix; cell surface; endoplasmic reticulum lumen

Molecular Function: integrin binding; protein binding; metallopeptidase activity; zinc ion binding; metalloendopeptidase activity; calcium ion binding

Biological Process: integrin-mediated signaling pathway; protein amino acid O-linked glycosylation; platelet activation; cellular protein metabolic process; glycoprotein metabolic process; response to toxin; cell-matrix adhesion; peptide catabolic process; protein processing; post-translational protein modification; proteolysis

Disease: Thrombotic Thrombocytopenic Purpura, Congenital