Rsk1, active, Rat Active Protein | Rps6ka1 active protein

Rsk1, active, Recombinant, Rat (Ribosomal S6 Kinase 1, RSK-1, MAP Kinase-activated Protein Kinase 1a, MAPK-activated Protein Kinase 1a, MAPKAP kinase 1a, Mapkapk1a, MAPKAPK-1a, 90kD Rbosomal Protein S6 Kinase 1, p90RSK1, p90-RSK 1, p90S6K, Ribosomal Prote

Cat. Num. AAA638214

• Purity: Highly Purified; ~95%. Purified using Ni2+/NTA-agarose. Activated with p42 MAPK and PDK1, and repurified by glutathione-, heparin-, and Ni2+/NTA-agarose chromatographies.
• Synonyms: Rsk1; active; Rat; Recombinant; Rat (Ribosomal S6 Kinase 1; RSK-1; MAP Kinase-activated Protein Kinase 1a; MAPK-activated Protein Kinase 1a; MAPKAP kinase 1a; Mapkapk1a; MAPKAPK-1a; 90kD Rbosomal Protein S6 Kinase 1; p90RSK1; p90-RSK 1; p90S6K; Ribosomal Prote; Rps6ka1 active protein

• Purity/Purification: Highly Purified; ~95%. Purified using Ni2+/NTA-agarose. Activated with p42 MAPK and PDK1, and repurified by glutathione-, heparin-, and Ni2+/NTA-agarose chromatographies.
• Form/Format: Supplied as a liquid in 50mM Tris-HCl, pH 7.5, 300mM sodium chloride, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol.

• Specific Activity: ~4000-5000U/mg, where one unit of RSK1 activity is defined as 1nmol phosphate incorporated into 30uM Rsktide per minute at 30 degree C with a final ATP concentration of 100uM.
• Preparation and Storage: Aliquot to avoid repeated freezing and thawing and store at -70 degree C. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Related Product Information for Rps6ka1 active protein

Recombinant full-length rat RSK1 (residues 1-end), containing additional N-terminal 6His and HA (YPYDVPDYA) tags. Expressed in baculovirus in Sf21 insect cells

Product Categories/Family for Rps6ka1 active protein

Molecular Biology; MB-Protein Kinases; Ribosomal

NCBI and Uniprot Product Information

• NCBI GI #: 13592065
• NCBI GeneID: 81771
• NCBI Accession #: NP_112369.1
• NCBI GenBank Nucleotide #: NM_031107.1
• UniProt Accession #: Q63531
• Molecular Weight: 89.9kD
• NCBI Official Full Name: ribosomal protein S6 kinase alpha-1
• NCBI Official Synonym Full Names: ribosomal protein S6 kinase polypeptide 1
• NCBI Official Symbol: Rps6ka1
• NCBI Protein Information: ribosomal protein S6 kinase alpha-1; RSK-1; p90S6K; p90RSK1; pp90RSK1; MAPKAPK1A; p90-RSK 1; MAPKAPK-1a; S6K-alpha 1; S6K-alpha-1; MAPKAP kinase 1a; ribosomal S6 kinase 1; S6 protein kinase (Rsk-1); MAPK-activated protein kinase 1a; 90 kDa ribosomal protein S6 kinase 1; MAP kinase-activated protein kinase 1a
• UniProt Protein Name: Ribosomal protein S6 kinase alpha-1
• Protein Family: Ribosomal protein S6 kinase
• UniProt Gene Name: Rps6ka1
• UniProt Synonym Gene Names: Mapkapk1a; Rsk1; S6K-alpha-1; p90-RSK 1; p90RSK1; p90S6K; MAPK-activated protein kinase 1a; MAPKAP kinase 1a; MAPKAPK-1a; RSK-1
• UniProt Entry Name: KS6A1_RAT

NCBI Description

catalyzes the phosphorylation of ETS transcription factor ER81 and other transcription factors [RGD, Feb 2006]

Uniprot Description

p90RSK: an AGC kinase of the RSK family. Phosphorylated and activated by Erk1 and -2 in response to many growth factors, polypeptide hormones and neurotransmitters. Several phosphorylation sites are important for its activation. Possesses two kinase domains connected by a regulator linker region. Phosphorylates a wide range of substrates including ribosomal protein S6. Prominently expressed in brain structures essential for cognitive function and learning.

Protein type: Translation; Protein kinase, Ser/Thr (non-receptor); Protein kinase, AGC; Kinase, protein; EC 2.7.11.1; AGC group; RSK family; RSK subfamily

Cellular Component: cytoplasm; ribosome; spindle; cytosol; nucleus

Molecular Function: protein serine/threonine kinase activity; caspase inhibitor activity; magnesium ion binding; protein serine/threonine/tyrosine kinase activity; ribosomal protein S6 kinase activity; transcription factor binding; ATP binding

Biological Process: positive regulation of transcription, DNA-dependent; positive regulation of transcription from RNA polymerase II promoter; negative regulation of caspase activity; protein amino acid phosphorylation; negative regulation of apoptosis