RANK Active Protein | TNFRSF11A active protein

RANK, Recombinant, Human (Receptor Activator of NFkB)

Cat. Num. AAA650880

• Gene Names: TNFRSF11A; FEO; OFE; ODFR; OSTS; PDB2; RANK; CD265; OPTB7; TRANCER; LOH18CR1
• Purity: Highly Purified; 98% by SDS-PAGE and HPLC analysis.
• Synonyms: RANK; Recombinant; Human (Receptor Activator of NFkB); TNFRSF11A active protein

• Purity/Purification: Highly Purified; 98% by SDS-PAGE and HPLC analysis.
• Form/Format: Supplied as a lyophilized powder in PBS, pH 7.6.

• Biological Activity: Determined by its ability to induce osteoclast formation in RAW264.7 cells using a concentration range of 5.0-10.0 ng/ml; The ED50 as determined by the dose-dependent stimulation of IL-8 production by human PBMC (peripheral blood mononuclear cells) is 10ng/ml.
• Endotoxin Level: 0.1ng/ug (1EU/ug)
• Reconstitution: Reconstitute with sterile dH2O to a concentration of 0.1-1mg/ml. Solution can then be diluted into other aqueous buffers and stored at 4 degree C for 1 week or -20 degree C for future use.
• Preparation and Storage: Lyophilized powder may be stored at 4 degree C for short-term only. Reconstitute to nominal volume by adding sterile dH2O, aliquot and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for TNFRSF11A active protein

Human RANK (receptor activator of NF-KappaB), a soluble 20kD protein, comprising the full-length of the TNF-like extracellular domain of RANKL, is a member of the TNFR family, identified as a dentritic cell membrane protein.

Product Categories/Family for TNFRSF11A active protein

Growth Factors; Cytokines; Growth Factors-RANK

NCBI and Uniprot Product Information

• NCBI GI #: 2612918
• NCBI GeneID: 8792
• UniProt Accession #: Q9Y6Q6; I4EC39
• Molecular Weight: 20kD
• NCBI Official Full Name: receptor activator of nuclear factor-kappa B
• NCBI Official Synonym Full Names: tumor necrosis factor receptor superfamily, member 11a, NFKB activator
• NCBI Official Symbol: TNFRSF11A
• NCBI Official Synonym Symbols: FEO; OFE; ODFR; OSTS; PDB2; RANK; CD265; OPTB7; TRANCER; LOH18CR1
• NCBI Protein Information: tumor necrosis factor receptor superfamily member 11A; receptor activator of NF-KB; osteoclast differentiation factor receptor; receptor activator of nuclear factor-kappa B; loss of heterozygosity, 18, chromosomal region 1
• UniProt Protein Name: Tumor necrosis factor receptor superfamily member 11A
• Protein Family: Tumor necrosis factor receptor superfamily
• UniProt Gene Name: TNFRSF11A
• UniProt Synonym Gene Names: RANK; ODFR
• UniProt Entry Name: TNR11_HUMAN

NCBI Description

The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptors can interact with various TRAF family proteins, through which this receptor induces the activation of NF-kappa B and MAPK8/JNK. This receptor and its ligand are important regulators of the interaction between T cells and dendritic cells. This receptor is also an essential mediator for osteoclast and lymph node development. Mutations at this locus have been associated with familial expansile osteolysis, autosomal recessive osteopetrosis, and Paget disease of bone. Alternatively spliced transcript variants have been described for this locus. [provided by RefSeq, Aug 2012]

Uniprot Description

Function: Receptor for TNFSF11/RANKL/TRANCE/OPGL; essential for RANKL-mediated osteoclastogenesis. Involved in the regulation of interactions between T-cells and dendritic cells. Ref.3

Subunit structure: Binds to the clefts between the subunits of the TNFSF11 ligand trimer to form a heterohexamer

By similarity. Interacts with TRAF1, TRAF2, TRAF3, TRAF5 and TRAF6. Interacts (via cytoplasmic domain) with GAB2. Ref.4 Ref.5

Subcellular location: Membrane; Single-pass type I membrane protein

Potential.

Tissue specificity: Ubiquitous expression with high levels in skeletal muscle, thymus, liver, colon, small intestine and adrenal gland.

Involvement in disease: Familial expansile osteolysis (FEO) [MIM:174810]: Rare autosomal dominant bone disorder characterized by focal areas of increased bone remodeling. The osteolytic lesions develop usually in the long bones during early adulthood. FEO is often associated with early-onset deafness and loss of dentition.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.7Paget disease of bone 2 (PDB2) [MIM:602080]: Bone-remodeling disorder with clinical similarities to FEO. Unlike FEO, however, affected individuals have involvement of the axial skeleton with lesions in the spine, pelvis and skull.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.7Osteopetrosis, autosomal recessive 7 (OPTB7) [MIM:612301]: A rare genetic disease characterized by abnormally dense bone, due to defective resorption of immature bone. Osteopetrosis occurs in two forms: a severe autosomal recessive form occurring in utero, infancy, or childhood, and a benign autosomal dominant form occurring in adolescence or adulthood. Recessive osteopetrosis commonly manifests in early infancy with macrocephaly, feeding difficulties, evolving blindness and deafness, bone marrow failure, severe anemia, and hepatosplenomegaly. Deafness and blindness are generally thought to represent effects of pressure on nerves. OPTB7 is characterized by paucity of osteoclasts, suggesting a molecular defect in osteoclast development. OPTB7 is associated with hypogammaglobulinemia.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.8

Sequence similarities: Contains 4 TNFR-Cys repeats.