Rabbit anti-Human IKB alpha Polyclonal Antibody | anti-IKBA antibody

Anti-Phospho-IKB alpha (S32) Rabbit pAb

Cat. Num. AAA4750063

• Gene Names: NFKBIA; IKBA; MAD-3; NFKBI
• Reactivity: Human
• Applications: Western Blot, Immunoprecipitation
• Purity: Affinity Chromatography
• Synonyms: IKB alpha; Polyclonal Antibody; Anti-Phospho-IKB alpha (S32) Rabbit pAb; I-kappa-B-alpha; IkappaBalpha; MAD3; NFKBI; NFKBIA; RL/IF-1; anti-IKBA antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Phospho-IKB alpha (S32) Antibody detects endogenous levels of total Phospho-IKB alpha (S32)
• Purity/Purification: Affinity Chromatography
• Form/Format: Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Sequence Length: 317

• Applicable Applications for anti-IKBA antibody: Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:2000; IP: 1:50
• Immunogen: A synthesized peptide derived from human Phospho-IKB alpha (S32)
• Subcellular Location: Cell membrane
• Preparation and Storage: Store at 4 degree C for short term.; Store at-20 degree C for long term.; Avoid freeze/thaw cycle.

Western Blot (WB)

(Western blot analysis of Phospho-IKB alpha (S32) expression in HeLa cell lysate treated with Calyculin A and TNF-a.)

Related Product Information for anti-IKBA antibody

Activation occurs via phosphorylation of IkappaBalpha at Ser32 and Ser36 followed by proteasome-mediated degradation that results in the release and nuclear translocation of active NF-kappaB. IkappaBalpha phosphorylation and resulting Rel-dependent transcription are activated by a highly diverse group of extracellular signals including inflammatory cytokines, growth factors, and chemokines. Kinases that phosphorylate IkappaB at these activating sites have been identified.

NCBI and Uniprot Product Information

• NCBI GI #: 10092619
• NCBI GeneID: 4792
• NCBI Accession #: NP_065390.1
• NCBI GenBank Nucleotide #: NM_020529.2
• UniProt Accession #: P25963; B2R8L6
• Molecular Weight: 39kDa
• NCBI Official Full Name: NF-kappa-B inhibitor alpha
• NCBI Official Synonym Full Names: NFKB inhibitor alpha
• NCBI Official Symbol: NFKBIA
• NCBI Official Synonym Symbols: IKBA; MAD-3; NFKBI
• NCBI Protein Information: NF-kappa-B inhibitor alpha
• UniProt Protein Name: NF-kappa-B inhibitor alpha
• UniProt Gene Name: NFKBIA
• UniProt Synonym Gene Names: IKBA; MAD3; NFKBI; IkB-alpha; IkappaBalpha

NCBI Description

This gene encodes a member of the NF-kappa-B inhibitor family, which contain multiple ankrin repeat domains. The encoded protein interacts with REL dimers to inhibit NF-kappa-B/REL complexes which are involved in inflammatory responses. The encoded protein moves between the cytoplasm and the nucleus via a nuclear localization signal and CRM1-mediated nuclear export. Mutations in this gene have been found in ectodermal dysplasia anhidrotic with T-cell immunodeficiency autosomal dominant disease. [provided by RefSeq, Aug 2011]

Uniprot Description

Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription.