PRDX1 active protein

PRDX1, Recombinant, Human (Peroxiredoxin-1, Natural Killer Cell-enhancing Factor A, NKEF-A, Proliferation-associated Gene Protein, PAG, Thioredoxin Peroxidase 2, Thioredoxin-dependent Peroxide Reductase 2, PAGA, PAGB, TDPX2)

Cat. Num. AAA635933

• Gene Names: PRDX1; PAG; PAGA; PAGB; PRX1; PRXI; MSP23; NKEFA; TDPX2; NKEF-A
• Purity: Highly Purified; ~95% (SDS-PAGE)
• Synonyms: PRDX1; Recombinant; Human (Peroxiredoxin-1; Natural Killer Cell-enhancing Factor A; NKEF-A; Proliferation-associated Gene Protein; PAG; Thioredoxin Peroxidase 2; Thioredoxin-dependent Peroxide Reductase 2; PAGA; PAGB; TDPX2); PRDX1 active protein

• Purity/Purification: Highly Purified; ~95% (SDS-PAGE)
• Form/Format: Supplied as a liquid in 20mM HEPES, pH 7.0, 1mM DTT, 10% glycerol.

• Specific Activity: 3.2U/mg (One unit will cause the oxidation of 1umole of NADPH at 25 degree C at pH 7.5)
• Preparation and Storage: Aliquot to avoid repeated freezing and thawing and store at -70 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Testing Data

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Related Product Information for PRDX1 active protein

Peroxiredoxin (Prx) is a growing peroxidase family, whose mammalian members have been known to connect with cell proliferation, differentiation, and apoptosis. Many isoforms (about 50 proteins), collected in accordance to the aa sequence homology, particularly amino-terminal region containing active site cysteine residue, and the thiol-specific antioxidant activity, distribute throughout all the kingdoms. Among them, mammalian Prx consists of 6 different members grouped into typical 2-Cys, atypical 2-Cys Prx, and 1-Cys Prx. Except Prx VI belonging to 1-Cys Prx subgroup, the other five 2-Cys Prx isotypes have the thioredoxin- dependent peroxidase (TPx) activity utilizing thioredoxin, thioredoxin reductase, and NADPH as a reducing system. Mammalian Prxs are 20-30kD in molecular size and vary in subcellular localization: Prx I, II, and VI in cytosol, Prx III in mitochondria, Prx IV in ER and secretion, Prx V showing complicated distribution including peroxisome, mitochondria and cytosol (1).

Product Categories/Family for PRDX1 active protein

Molecular Biology; MB-Enzymes; Peroxiredoxin

NCBI and Uniprot Product Information

• NCBI GI #: 320461711
• NCBI GeneID: 5052
• NCBI Accession #: NP_001189360.1
• NCBI GenBank Nucleotide #: NM_001202431.1
• UniProt Accession #: Q06830; P35703; Q2V576; Q5T154; Q5T155; B5BU26; D3DPZ8
• Molecular Weight: ~22kD
• NCBI Official Full Name: peroxiredoxin-1
• NCBI Official Synonym Full Names: peroxiredoxin 1
• NCBI Official Symbol: PRDX1
• NCBI Official Synonym Symbols: PAG; PAGA; PAGB; PRX1; PRXI; MSP23; NKEFA; TDPX2; NKEF-A
• NCBI Protein Information: peroxiredoxin-1; thioredoxin peroxidase 2; proliferation-associated gene A; natural killer-enhancing factor A; proliferation-associated gene protein; natural killer cell-enhancing factor A; thioredoxin-dependent peroxide reductase 2
• UniProt Protein Name: Peroxiredoxin-1
• Protein Family: Peroxiredoxin
• UniProt Gene Name: PRDX1
• UniProt Synonym Gene Names: PAGA; PAGB; TDPX2; NKEF-A; PAG
• UniProt Entry Name: PRDX1_HUMAN

NCBI Description

This gene encodes a member of the peroxiredoxin family of antioxidant enzymes, which reduce hydrogen peroxide and alkyl hydroperoxides. The encoded protein may play an antioxidant protective role in cells, and may contribute to the antiviral activity of CD8(+) T-cells. This protein may have a proliferative effect and play a role in cancer development or progression. Four transcript variants encoding the same protein have been identified for this gene. [provided by RefSeq, Jan 2011]

Uniprot Description

PRDX1: a member of the peroxiredoxin family of antioxidant enzymes, which reduce hydrogen peroxide and alkyl hydroperoxides. Involved in redox regulation of the cell. Reduces peroxides with reducing equivalents provided through the thioredoxin system but not from glutaredoxin. May play an important role in eliminating peroxides generated during metabolism. Might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H(2)O(2). May have a proliferative effect and play a role in cancer development or progression.

Protein type: Oxidoreductase; EC 1.11.1.15; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 1p34.1

Cellular Component: peroxisomal matrix; extracellular space; mitochondrial matrix; cytoplasm; melanosome; nucleolus; cytosol; nucleus

Molecular Function: protein binding; peroxidase activity; protein homodimerization activity; thioredoxin peroxidase activity; heme binding

Biological Process: regulation of stress-activated MAPK cascade; retinal homeostasis; cell proliferation; erythrocyte homeostasis; response to reactive oxygen species; removal of superoxide radicals; hydrogen peroxide catabolic process; natural killer cell mediated cytotoxicity; skeletal development; regulation of NF-kappaB import into nucleus