ASF1A recombinant protein

ASF1A, 1-204aa, Human, His tag, E Coli

Cat. Num. AAA203216

• Gene Names: ASF1A; CIA; CGI-98; HSPC146
• Applications: SDS-Page
• Purity: > 90% by SDS-PAGE
• Synonyms: ASF1A; 1-204aa; Human; His tag; E Coli; ASF1 anti-silencing function 1 homolog A; Histone chaperone ASF1A; CCG1-interacting factor A (CIA); CGI-98; HSPC146; ASF1 anti-silencing function 1 homolog A Anti silencing function 1A; ASF1 anti silencing function 1 homolog A; CCG1 interacting factor A; CGI 98; CIA; hAsf1; hAsf1a; hCIA; ; ASF1A recombinant protein

• Host: E Coli
• Purity/Purification: > 90% by SDS-PAGE
• Form/Format: Liquid. In 20 mM Tris-HCl Buffer (pH 8.0) containing 1mM DTT, 10% glycerol
• Concentration: 1 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMAKV QVNNVVVLDN PSPFYNPFQF EITFECIEDL SEDLEWKIIY VGSAESEEYD QVLDSVLVGP VPAGRHMFVF QADAPNPGLI PDADAVGVTV VLITCTYRGQ EFIRVGYYVN NEYTETELRE NPPVKPDFSK LQRNILASNP RVTRFHINWE DNTEKLEDAE SSNPNLQSLL STDALPSASK GWSTSENSLN VMLESHMDCM
• Sequence Length: 204

• Applicable Applications for ASF1A recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Endotoxin Level: < 1.0 EU per 1 microgram of protein (determined by LAL method)
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for ASF1A recombinant protein

Histone chaperone ASF1A, CCG1-interacting factor A (CIA), CGI-98, HSPC146 Description ASF1 anti-silencing function 1 homolog A, also known as ASF1A, belongs to H3/H4 family of histone chaperone proteins. ASF1A protein interacts with histones H3 and H4, and functions together with a chromatin assembly factor during DNA replication and repair. Deletion of ASF1A in yeast and Drosophila confers sensitivity to various DNA damaging agents and inhibitors of DNA replication, increases genomic instability and sister chromatid exchange, and activates the DNA damage checkpoint. Recombinant human ASF1A, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography.

Product Categories/Family for ASF1A recombinant protein

Histone Related

References

Sanematsu F., et al. (2006). J Biol Chem. 281 (19):13817-27; Groth A., et al. (2005). Mol Cell. 17 (2):301-11;

NCBI and Uniprot Product Information

• NCBI GI #: 7661592
• NCBI GeneID: 25842
• NCBI Accession #: NP_054753
• NCBI GenBank Nucleotide #: NM_014034.2
• UniProt Accession #: Q9Y294; Q6IA08; Q9P014
• Molecular Weight: 27 kDa (240aa), confirmed by MALDI-TOF.
• NCBI Official Full Name: histone chaperone ASF1A
• NCBI Official Synonym Full Names: anti-silencing function 1A histone chaperone
• NCBI Official Symbol: ASF1A
• NCBI Official Synonym Symbols: CIA; CGI-98; HSPC146
• NCBI Protein Information: histone chaperone ASF1A
• UniProt Protein Name: Histone chaperone ASF1A
• Protein Family: Histone chaperone
• UniProt Gene Name: ASF1A
• UniProt Synonym Gene Names: hAsf1; hAsf1a; CIA; hCIA

NCBI Description

This gene encodes a member of the H3/H4 family of histone chaperone proteins and is similar to the anti-silencing function-1 gene in yeast. The protein is a key component of a histone donor complex that functions in nucleosome assembly. It interacts with histones H3 and H4, and functions together with a chromatin assembly factor during DNA replication and repair. [provided by RefSeq, Jul 2008]

Uniprot Description

Histone chaperone that facilitates histone deposition and histone exchange and removal during nucleosome assembly and disassembly. Cooperates with chromatin assembly factor 1 (CAF-1) to promote replication-dependent chromatin assembly and with HIRA to promote replication-independent chromatin assembly. Required for the formation of senescence-associated heterochromatin foci (SAHF) and efficient senescence-associated cell cycle exit.