LRPAP Active Protein | Lrpap1 active protein
LRPAP, Recombinant, Human (LDL Receptor-related Protein-Associated Protein 1, A2MRAP, A2RAP, Alpha-2-macroglobulin receptor-associated protein, Alpha-2-MRAP, HBP44, MGC138272, MRAP, RAP)
Purity
Highly Purified90%, as determined by SDS-PAGE under reducing conditions and visualized by silver stain.
Synonyms
LRPAP; Recombinant; Human (LDL Receptor-related Protein-Associated Protein 1; A2MRAP; A2RAP; Alpha-2-macroglobulin receptor-associated protein; Alpha-2-MRAP; HBP44; MGC138272; MRAP; RAP); Lrpap1 active protein
Purity/Purification
Highly Purified
90%, as determined by SDS-PAGE under reducing conditions and visualized by silver stain.
90%, as determined by SDS-PAGE under reducing conditions and visualized by silver stain.
Form/Format
Supplied as a lyophilized powder in PBS. Reconstitute with sterile PBS to 0.1mg/ml.
Activity
Measured by its binding ability in a functional ELISA. Immobilized human LRPAP at 0.5ug/ml can bind rmVLDLR with an apparent KD < 0.25nM.
Endotoxin Level
1EU/ug protein (LAL method)
Preparation and Storage
Lyophilized powder stored at -20 degree C to -70 degree C is stable for 12 months. Reconstituted product under sterile conditions is stable for 3 months at -20 degree C to -70 degree C. Reconstituted product under sterile conditions is stable for 1 month at 4 degree C.
Related Product Information for Lrpap1 active protein
LRPAP (LDL receptor-related protein-associated protein 1; also RAP) is a ubiquitously expressed 39kD molecular chaperone for LDL receptor family proteins (1, 2). Mature mouse LRPAP is 332 amino acids (aa) in length and secreted into the ER/Golgi of the cell. It shares 77% and 97% aa sequence identity with human and rat LRPAP, respectively. LRPAP contains three approximately 100 aa alpha-helical domains (D1-D3). The D1 domain contains a low affinity binding site for LRP, and the associated D2 and D3 domains bind LRP with high affinity (4). Domains D2 and D3 interact with each other, while D1 is independent (3). The majority of LRPAP is localized in the endoplasmic reticulum and Golgi (5). LRPAP prevents the premature interaction of LRP, LRP2/megalin, and VLDLR with their coexpressed ligands, thereby promoting proper receptor folding and export from the ER (6-8). Protonation of conserved histidine residues within the D3 domain induces the separation of LRPAP and LRP in the relatively acidic Golgi (9). LRPAP, which contains a C-terminal HNEL motif, can then recycle to the ER (9). A minor amount of LRPAP remains associated with LRP and can modulate receptor activity on the cell surface (5). Exogenously applied LRPAP competitively inhibits LDL receptor family binding and uptake of activated alpha 2-macroglobulin, apoB100- or apoE-enriched LDL and VLDL particles, cholesteryl esters, and complexes of PAI-1 with either tPA or uPA (10-14).
Product Categories/Family for Lrpap1 active protein
NCBI and Uniprot Product Information
NCBI GI #
UniProt Accession #
Molecular Weight
38.7kD
NCBI Official Full Name
low density lipoprotein receptor-associated protein 1
UniProt Protein Name
Low density lipoprotein receptor-associated protein 1
Protein Family
UniProt Gene Name
Lrpap1
UniProt Synonym Gene Names
lrpap1
UniProt Entry Name
Q6ZY49_MOUSE
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Product Notes
The LRPAP lrpap1 (Catalog #AAA635871) is an Active Protein and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "LRPAP, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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