LRPAP1 active protein

LRPAP1 Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8120686

• Gene Names: LRPAP1; RAP; MRAP; A2RAP; HBP44; A2MRAP
• Purity: >92% as determined by SDS-PAGE
• Synonyms: LRPAP1; LRPAP1 Protein; Human; Recombinant (His Tag); Human LRPAP1/A2MRAP Protein (His Tag); A2MRAP Protein; A2RAP Protein; alpha-2-MRAP Protein; HBP44 Protein; MRAP Protein; MYP23 Protein; RAP Protein; low density lipoprotein receptor-related protein associated protein 1; LRPAP1 active protein

• Host: HEK293 Cells
• Purity/Purification: >92% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Tyr35-Leu357

• Species: Human
• Activity: Measured by its binding ability in a functional ELISA. Immobilized human LRPAP1 at 0.5ug/ml can bind human VLDLR with a range of 3.2-400ng/mL.
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Tyr 35
• Tag: C-His
• Protein Construction: A DNA sequence encoding the human LRPAP1 (NP_002328.1)(Tyr 35-Leu 357) was expressed, fused with a signal peptide at the N-terminus and a polyhistidine tag at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Testing Data

(Measured by its binding ability in a functional ELISA. Immobilized human LRPAP1 at 0.5 ug/ml can bind human VLDLR with a range of 3.2-400 ng/ml.)

Related Product Information for LRPAP1 active protein

Background: Receptor-associated protein (RAP) is a molecular chaperone for low-density lipoprotein receptor-related protein (LRP), which plays a key role in cholesterol metabolism. The lipoprotein receptor-related protein (LRP) is an endocytic receptor for several ligands, such as alpha2-macroglobulin (alpha2 M) and apolipoprotein E. LRP is involved in the clearance of lipids from the bloodstream and is expressed in the atherosclerotic plaque. The LRP-associated protein (LRPAP in humans, RAP in mice) acts as a chaperone protein, stabilizing the nascent LRP peptide in the endoplasmic reticulum and Golgi complex. Alpha-2-macroglobulin receptor-associated protein, also known as low-density lipoprotein receptor-related protein-associated protein 1, RAP, and LRPAP1, is a 39kDa protein and a member of the alpha-2-MRAP family. It is a receptor antagonist that interacts with several members of the low-density lipoprotein (LDL) receptor gene family. Upon binding to these receptors, LRPAP1 inhibits all ligand interactions with the receptors. LRPAP1 is present on the cell surface forming a complex with the alpha-2-macroglobulin receptor heavy and light chains. It binds with LRP1B and the binding is followed by internalization and degradation. LRPAP1 interacts with LRP1/alpha-2-macroglobulin receptor and LRP2 (previously called glycoprotein 330) and may be involved in the pathogenesis of membrane glomerular nephritis. LRPAP1 together with LRP2 forms the Heymann nephritis antigenic complex. LRP2 is expressed in epithelial cells of the thyroid, where it can serve as a receptor for the protein thyroglobulin. Intron 5 insertion/deletion polymorphism of RAP gene (LRPAP1) has been implicated in other diseases sharing etiology with gallstone disease (GSD). The LRPAP1 insertion/deletion polymorphism influences cholesterol homeostasis and may confer risk for gallstone disease and gallbladder carcinoma (GBC) incidence usually parallels with the prevalence of cholelithiasis. The genetic variations at the LRPAP1 locus may modulate Alzheimer's disease (AD) phenotype beyond risk for disease. Also, the variation in the LRPAP1 gene could contribute to the risk of developing an early episode of myocardial infarction (MI).

References

Gonzlez P, et al. (2002) Variation in the lipoprotein receptor-related protein, alpha2-macroglobulin and lipoprotein receptor-associated protein genes in relation to plasma lipid levels and risk of early myocardial infarction. Coron Artery Dis. 13(5): 251-4.Schutte DL, et al. (2003) A LRPAP1 intronic insertion/deletion polymorphism and phenotypic variability in Alzheimer disease. Res Theory Nurs Pract. 17(4): 301-19Pandey SN, et al. (2006) Lipoprotein receptor associated protein (LRPAP1) insertion/deletion polymorphism: association with gallbladder cancer susceptibility. Int J Gastrointest Cancer. 37(4): 124-8.Dixit M, et al. (2006) Association of low density lipoprotein receptor related protein-associated protein (LRPAP1) gene insertion/deletion polymorphism with gallstone disease.J Gastroenterol Hepatol. 21(5): 847-9.

NCBI and Uniprot Product Information

• NCBI GI #: 4505021
• NCBI GeneID: 4043
• NCBI Accession #: NP_002328.1
• NCBI GenBank Nucleotide #: NM_002337.3
• UniProt Accession #: P30533; Q2M310; Q53HQ3; Q53HS6; D3DVR9
• Molecular Weight: 160,000
• NCBI Official Full Name: alpha-2-macroglobulin receptor-associated protein
• NCBI Official Synonym Full Names: low density lipoprotein receptor-related protein associated protein 1
• NCBI Official Symbol: LRPAP1
• NCBI Official Synonym Symbols: RAP; MRAP; A2RAP; HBP44; A2MRAP
• NCBI Protein Information: alpha-2-macroglobulin receptor-associated protein; alpha-2-MRAP; lipoprotein receptor associated protein; alpha-2-macroglobulin receptor-associated protein 1; low density lipoprotein receptor-related protein-associated protein 1; low density lipoprotein-r
• UniProt Protein Name: Alpha-2-macroglobulin receptor-associated protein
• Protein Family: Alpha-2-macroglobulin receptor-associated protein
• UniProt Gene Name: LRPAP1
• UniProt Synonym Gene Names: A2MRAP; Alpha-2-MRAP; RAP
• UniProt Entry Name: AMRP_HUMAN

NCBI Description

This gene encodes a protein that interacts with the low density lipoprotein (LDL) receptor-related protein and facilitates its proper folding and localization by preventing the binding of ligands. Mutations in this gene have been identified in individuals with myopia 23. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2013]

Uniprot Description

LRPAP1: Interacts with LRP1/alpha-2-macroglobulin receptor and glycoprotein 330. In complex with the alpha-2-MR or gp330, it may have some role in the pathogenesis of membrane glomerular nephritis. Belongs to the alpha-2-MRAP family.

Protein type: Receptor, misc.

Chromosomal Location of Human Ortholog: 4p16.3

Cellular Component: cell surface; endoplasmic reticulum; extracellular region; plasma membrane; integral to membrane; vesicle

Molecular Function: heparin binding; receptor antagonist activity; protein binding; low-density lipoprotein receptor binding; unfolded protein binding; asialoglycoprotein receptor activity

Biological Process: vesicle-mediated transport; receptor-mediated endocytosis; protein folding; negative regulation of protein binding

Disease: Myopia 23, Autosomal Recessive