AcCoA Carboxylase beta, C-terminal HIS tag Active Protein

AcCoA Carboxylase beta, C-terminal HIS tag, Recombinant, Human (ACACB, acetyl-Coenzyme A carboxylase beta, ACC2, ACCB, ACC-beta, Acetyl-CoA carboxylase 2, HACC275)

Cat. Num. AAA638282

• Purity: >=30%
• Synonyms: AcCoA Carboxylase beta; C-terminal HIS tag; Recombinant; Human (ACACB; acetyl-Coenzyme A carboxylase beta; ACC2; ACCB; ACC-beta; Acetyl-CoA carboxylase 2; HACC275); C-terminal HIS tag active protein

• Purity/Purification: >=30%
• Form/Format: Supplied as a liquid in 45mM Tris-HCl, pH 8.0, 500mM sodium chloride, 2.2mM KCl, 3mM DTT, variable amounts of Imidazole, 10% glycerol.
• Concentration: 0.07mg/ml (varies by lot)

• Application Notes: Suitable for use in the study of enzyme kinetics, screening inhibitors and selectivity profiling.; Optimal dilutions to be determined by the researcher.
• Specific Activity: >=42 pmol/min/ug.; A 25ul ACC2 reaction is conducted in a buffer containing 30mM HEPES, pH 7.4, 2mM MgCl2, 2mM sodium citrate, 1mM DTT 100uM ATP, 12mM NaHCO3 and 20uM acetyl-CoA at 30 degree C for 45 minutes. ATP reduction is detected using Kinase-Glo Luminescent Kinase Assay Platform.
• Source: Recombinant corresponding to full length human ACC2 with C-terminal His tag expressed in Baculovirus infected Sf9 cell (NM_001093).
• Preparation and Storage: Aliquot to avoid repeated freezing and thawing. Store at -70 degree C. Aliquots are stable for at least 6 months at -70 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.; Shipping: Ships with Dry Ice

Testing Data

(Specific activity.)

SDS-Page

(10% SDS-PAGE Coomassie using A0225-08. Lane 1: 7ug of ACACB. Lane 2: Protein Marker)

Product Categories/Family for AcCoA Carboxylase beta, C-terminal HIS tag active protein

Molecular Biology; MB-CoEnzyme A

NCBI and Uniprot Product Information

• Molecular Weight: 277kD

Uniprot Description

Catalyzes the ATP-dependent carboxylation of acetyl-CoA to malonyl-CoA. Carries out three functions: biotin carboxyl carrier protein, biotin carboxylase and carboxyltransferase. Involved in inhibition of fatty acid and glucose oxidation and enhancement of fat storage (). May play a role in regulation of mitochondrial fatty acid oxidation through malonyl-CoA-dependent inhibition of carnitine palmitoyltransferase 1 ().