PDE3B active protein

PDE3B, Recombinant, Human (Phosphodiesterase 3B, cGMP-inhibited, CGIP1, cGIPDE1, CGIPDE1, CGI-PDE B, cGMP-inhibited 3',5'-cyclic Phosphodiesterase B, Cyclic GMP-inhibited Phosphodiesterase B, HcGIP1)

Cat. Num. AAA635538

• Gene Names: PDE3B; HcGIP1; cGIPDE1
• Purity: Purified; 0.36
• Synonyms: PDE3B; Recombinant; Human (Phosphodiesterase 3B; cGMP-inhibited; CGIP1; cGIPDE1; CGIPDE1; CGI-PDE B; cGMP-inhibited 3'; 5'-cyclic Phosphodiesterase B; Cyclic GMP-inhibited Phosphodiesterase B; HcGIP1); PDE3B active protein

• Purity/Purification: Purified; 0.36
• Form/Format: Supplied as a liquid in 40mM Tris, pH 8.0, 110mM NaCl, 2.2mM KCl, 3mM DTT, 0.04%

• Application Notes: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
• Specific Activity: Lot-specific. Sample lot value: 1270pmol/min/ug
• Unit Definition: 1 unit is defined as the amount of enzyme that will convert 1 pmole of 3', 5'-cAMP to 5' AMP per min at 30 degree C in a reaction buffer.
• Preparation and Storage: Store at -70 degree C. Stable for at least 6 months. Avoid freeze-thaw cycles. or maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Testing Data

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Related Product Information for PDE3B active protein

Cyclic nucleotides are hydrolyzed and compartmentalized by a family of enzymes called phosphodieterases. In mammals at least 11 different families of PDEs can be discriminated (PDE1-PDE11) based on their kinetic properties and inhibition to various pharmacological agents. The members of the PDE3 families are known as cGMP inhibited PDEs. The PDE3 enzymes bind both cGMP and cAMP with different affinities (cAMP 4-10-fold than cGMP). The phosphodiesterase type-3 (PDE3) family is comprised of 2 genes (PDE3A and PDE3B). PDE3A is a 145kD protein and is expressed in various tissues (myocardium, vascular and non vascular smooth muscles, megakaryocytes and a subset of neurons (Snyder 1999; Maecci et al., 1992). PDE3B is 1112aa long and has two splice variants which are expressed in adipocyte tissue, hepatocyte, kidney, spermatocytes and embryonic neuroepithelium (Reinhatdt et. al., 1995). Both PDE3A and PDE3B are therapeutic targets for the treatment of obesity as inhibition of PDE3 increase the lipolytic activity and alter the insulin secretion and its response. PDE3 inhibitors also promote thermogenic and cardiotonic effects in animals.

Product Categories/Family for PDE3B active protein

Molecular Biology; MB-Enzymes; Phosphodiesterase

NCBI and Uniprot Product Information

• NCBI GI #: 219519624
• NCBI GeneID: 5140
• UniProt Accession #: Q13370; O00639; Q14408; Q6SEI4
• Molecular Weight: 86kD
• NCBI Official Full Name: PDE3B protein
• NCBI Official Synonym Full Names: phosphodiesterase 3B, cGMP-inhibited
• NCBI Official Symbol: PDE3B
• NCBI Official Synonym Symbols: HcGIP1; cGIPDE1
• NCBI Protein Information: cGMP-inhibited 3',5'-cyclic phosphodiesterase B; CGIP1; CGI-PDE B; cyclic nucleotide phosphodiesterase; cyclic GMP-inhibited phosphodiesterase B
• UniProt Protein Name: cGMP-inhibited 3',5'-cyclic phosphodiesterase B
• Protein Family: cGMP-inhibited 3',5'-cyclic phosphodiesterase
• UniProt Gene Name: PDE3B
• UniProt Synonym Gene Names: CGIP1; CGI-PDE B
• UniProt Entry Name: PDE3B_HUMAN

Uniprot Description

PDE3B: a cGMP-inhibited phosphodiesterase 1 involved in fat metabolism. Inhibition of PDE3B can augment PDE4 inhibitor-induced apoptosis in some patients with chronic lymphocytic leukemia.

Protein type: Membrane protein, integral; Nucleotide Metabolism - purine; Phosphodiesterase; Membrane protein, multi-pass; EC 3.1.4.17

Chromosomal Location of Human Ortholog: 11p15.1

Cellular Component: Golgi apparatus; membrane; endoplasmic reticulum; integral to membrane; cytosol

Molecular Function: protein kinase B binding; protein binding; cGMP-inhibited cyclic-nucleotide phosphodiesterase activity; metal ion binding; 3',5'-cyclic-nucleotide phosphodiesterase activity

Biological Process: negative regulation of angiogenesis; cellular response to insulin stimulus; negative regulation of lipid catabolic process; cAMP catabolic process; insulin receptor signaling pathway; negative regulation of cell adhesion; angiogenesis; glucose homeostasis; blood coagulation; regulation of insulin secretion; endocrine pancreas development; negative regulation of cell adhesion mediated by integrin