Testing Data
(specific activity)
Protein Kinase G1 beta, N-terminal HIS tag Active Protein
Protein Kinase G1 beta, N-terminal HIS tag, Recombinant, Human (PKG1 beta, Cyclic GMP-dependent Protein Kinase)
Purity
PurifiedPurified ~70%
Synonyms
Protein Kinase G1 beta; N-terminal HIS tag; Recombinant; Human (PKG1 beta; Cyclic GMP-dependent Protein Kinase); N-terminal HIS tag active protein
Purity/Purification
Purified
Purified ~70%
Purified ~70%
Form/Format
Supplied as a liquid in 25mM Tris-HCl, pH 7.5, 100mM sodium chloride, 0.05% tween 20, 3mM DTT, 20% glycerol.
Application Notes
Suitable for use in the study of enzyme kinetics, screening inhibitors and selectivity profiling.
Specific Activity
0.85pmoles/min/ug. Enzyme reaction is conducted in a buffer containing 50mM HEPES, pH 7.5, 10mM magnesium chloride, 1mM EGTA, 200uM ATP, 0.01% Brij-35, 2uM substrate (SER/THR 14) at RT for 1hour.
Preparation and Storage
Aliquot to avoid repeated freezing and thawing. Store at -70 degree C. Aliquots are stable for at least 12 months at -70 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Testing Data
(specific activity)
Testing Data
(specific activity)
SDS-Page
(10% SDS page coomassie staining using P9102-95R. Lane 1: 3ug PKG1 beta. Lane 2: Protein marker.)
SDS-Page
(10% SDS page coomassie staining using P9102-95R. Lane 1: 3ug PKG1 beta. Lane 2: Protein marker.)
Product Categories/Family for Protein Kinase G1 beta, N-terminal HIS tag active protein
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Product Notes
The Protein Kinase G1 beta, N-terminal HIS tag (Catalog #AAA638140) is an Active Protein and is intended for research purposes only. The product is available for immediate purchase. Suitable for use in the study of enzyme kinetics, screening inhibitors and selectivity profiling. Researchers should empirically determine the suitability of the Protein Kinase G1 beta, N-terminal HIS tag for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Protein Kinase G1 beta, N-terminal HIS tag, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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