PI 3-Kinase p110 beta/p85 alpha Active Protein | PIK3R1 active protein

PI 3-Kinase p110 beta/p85 alpha, Recombinant, Human

Cat. Num. AAA636545

• Gene Names: PIK3R1; p85; AGM7; GRB1; p85-ALPHA
• Purity: Purified; 0.7
• Synonyms: PI 3-Kinase p110 beta/p85 alpha; Recombinant; Human; PIK3R1 active protein

• Purity/Purification: Purified; 0.7
• Form/Format: Supplied as a liquid in 25mM Tris-HCl, pH 8.0, 100mM NaCl, 0.05% Tween-20, 50% glycerol, 3mM DTT.

• Application Notes: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.; Dilution: A 50ul kinase reaction is conducted in a buffer containing 10mM Tris-HCl, pH 7.5,; 50mM NaCl, 5mM MgCl2, 5uM ATP, 17ug Phosphoinositol-4,5-Bisphosphate, and 150ng enzyme for 90 minutes at room temperature. ATP reduction is detected using Kinase-Glo® Luminescent Kinase Assay Platform.
• Specific Activity: 7.9pmol/min/mg
• Preparation and Storage: Aliquot to avoid repeated freezing and thawing and store at -70 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 6 months.

Testing Data

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Testing Data

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Related Product Information for PIK3R1 active protein

The PI3Kb isoform can be activated by insulin via the insulin receptor to initiate a cascade of events that control cell growth and metabolism. The activation of PI3Kb is mediated by the p85 regulatory subunit binding to tyrosine phosphorylated insulin receptor substrate (IRS) proteins (e.g. IRS-1 and IRS-2). It was also shown that PI3Kb is involved in apoptosis in human colon carcinoma cells. Injection of neutralizing antibodies specific to p110b in WiDr, HCT116 and CO 115 adenocarcinoma cells inhibited de novo DNA synthesis. PI3Kb is the major PI3K isoform required for apoptotic cell and Fc-g receptor mediated phagocytosis shown for primary mouse macrophages and the Jurkat human leukemia T cell line. It was shown by several research groups that the catalytic subunit of PI3Kb can be activated by Gbg subunits of G-protein coupled receptors.

Product Categories/Family for PIK3R1 active protein

Molecular Biology; MB-Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 32455252
• NCBI GeneID: 5295
• NCBI Accession #: NP_852556.2
• NCBI GenBank Nucleotide #: NM_181504.3
• UniProt Accession #: P27986; Q15747; Q4VBZ7; Q53EM6; Q8IXA2; Q8N1C5; B3KT19; D3DWA0; E7EX19
• Molecular Weight: 124kD, 83.7kD
• NCBI Official Full Name: phosphatidylinositol 3-kinase regulatory subunit alpha isoform 2
• NCBI Official Synonym Full Names: phosphoinositide-3-kinase, regulatory subunit 1 (alpha)
• NCBI Official Symbol: PIK3R1
• NCBI Official Synonym Symbols: p85; AGM7; GRB1; p85-ALPHA
• NCBI Protein Information: phosphatidylinositol 3-kinase regulatory subunit alpha; PI3-kinase subunit p85-alpha; PI3K regulatory subunit alpha; ptdIns-3-kinase regulatory subunit alpha; phosphoinositide-3-kinase regulatory subunit; phosphatidylinositol 3-kinase-associated p-85 alpha; phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha; phosphatidylinositol 3-kinase, regulatory subunit, polypeptide 1 (p85 alpha)
• UniProt Protein Name: Phosphatidylinositol 3-kinase regulatory subunit alpha
• Protein Family: Phosphatidylinositol 3-kinase regulatory
• UniProt Gene Name: PIK3R1
• UniProt Synonym Gene Names: GRB1; PI3-kinase regulatory subunit alpha; PI3K regulatory subunit alpha; PtdIns-3-kinase regulatory subunit alpha; PI3-kinase subunit p85-alpha
• UniProt Entry Name: P85A_HUMAN

NCBI Description

Phosphatidylinositol 3-kinase phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. The enzyme comprises a 110 kD catalytic subunit and a regulatory subunit of either 85, 55, or 50 kD. This gene encodes the 85 kD regulatory subunit. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance. Alternative splicing of this gene results in four transcript variants encoding different isoforms. [provided by RefSeq, Jun 2011]

Uniprot Description

PIK3R1: regulatory subunit of phosphoinositide-3-kinase. Mediates binding to a subset of tyrosine-phosphorylated proteins through its SH2 domain. Acts as an adapter, mediating the association of the p110 catalytic unit of the alpha, beta and delta enzymes to the plasma membrane, where p110 phosphorylates inositol lipids. May play an additional role in the regulation of the actin cytoskeleton. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. Its SH2 domains interacts with the YTHM motif of phosphorylated INSR in vitro. Defects in PIK3R1 are a cause of severe insulin resistance. Four alternatively spliced isoforms have been described.

Protein type: Motility/polarity/chemotaxis; Kinase, lipid; Enzyme, regulatory subunit

Chromosomal Location of Human Ortholog: 5q13.1

Cellular Component: membrane; cytoplasm; plasma membrane; intercellular junction; phosphoinositide 3-kinase complex; cytosol; nucleus

Molecular Function: insulin binding; insulin-like growth factor receptor binding; protein binding; ErbB-3 class receptor binding; neurotrophin TRKA receptor binding; insulin receptor substrate binding; phosphoinositide 3-kinase binding; phosphoinositide 3-kinase regulator activity; transcription factor binding; transmembrane receptor protein tyrosine kinase adaptor protein activity; protein phosphatase binding; insulin receptor binding

Biological Process: negative regulation of osteoclast differentiation; phosphoinositide 3-kinase cascade; nerve growth factor receptor signaling pathway; viral reproduction; T cell receptor signaling pathway; protein amino acid phosphorylation; positive regulation of RNA splicing; NFAT protein import into nucleus; regulation of phosphoinositide 3-kinase activity; positive regulation of glucose import; phosphoinositide phosphorylation; phospholipid metabolic process; positive regulation of transcription factor import into nucleus; epidermal growth factor receptor signaling pathway; platelet activation; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; protein stabilization; negative regulation of cell-matrix adhesion; positive regulation of tumor necrosis factor production; DNA damage response, signal transduction resulting in induction of apoptosis; cellular response to insulin stimulus; induction of apoptosis via death domain receptors; insulin-like growth factor receptor signaling pathway; B cell differentiation; T cell costimulation; phosphatidylinositol biosynthetic process; insulin receptor signaling pathway; cell glucose homeostasis; innate immune response; positive regulation of transcription from RNA polymerase II promoter; vascular endothelial growth factor receptor signaling pathway; blood coagulation; leukocyte migration; positive regulation of cell migration; negative regulation of apoptosis

Disease: Agammaglobulinemia 7, Autosomal Recessive; Short Syndrome; Immunodeficiency 36